Application of FOXE1 gene and WNT3 gene as well as methylation amplimer and probe of FOXE1 gene and WNT3 gene
An amplification primer and methylation technology, applied in the field of methylation amplification primers and probes, can solve the problem of not finding tumor markers, etc.
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[0017] 1. Materials and methods:
[0018] 1. Materials: normal colorectal mucosa (approximately 50 cases) were obtained from colonoscopic biopsy specimens, colorectal cancer tissues and adjacent normal tissues (approximately 300 cases) were obtained from surgical resection specimens, and all cases were newly diagnosed patients. Whole blood, serum and plasma from patients with colorectal cancer (312 cases) and normal controls (200 cases) were collected without radiotherapy and chemotherapy and confirmed by pathology. Patients with colorectal cancer included 181 males and 131 females. Aged 38 - 82 years old, with an average of 61.6 ± 12.3 years old. 200 healthy subjects were selected as the normal control group, including 118 males and 82 females. Aged 21-71 years old, average age 40.21 ± 18.05 years old. Specimens were obtained with the informed consent of the patients and approved by the Medical Ethics Committee of Run Run Shaw Hospital.
[0019] 2. Method:
[0020] 2.1 D...
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