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Extraction method of cationic anthocyanidin aglycone

An extraction method and anthocyanin technology, which are applied in the field of extraction of cationic anthocyanin aglycone, can solve problems such as microbial air pollution, complex production equipment, and harm to human health, and achieve pollution avoidance, low production cost, and high production efficiency high effect

Inactive Publication Date: 2014-06-04
耿兆翔
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Problems solved by technology

However, it uses microorganisms to separate anthocyanins from water-insoluble anthocyanin salt compounds. The production process is long, it takes a long time, the production equipment is more complicated, the production cost is high, and the production efficiency is low. There may also be microorganisms. Pollution to the air environment, endangering human health

Method used

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Embodiment

[0039] When extracting, proceed as follows:

[0040] 1. Pretreatment of raw materials: Fresh raw materials containing plant anthocyanins, such as carrots, are crushed → soaked in soft water to absorb water → frozen to destroy the cell wall of plant tissue → heated and thawed → squeezed → filtered for juice preparation (1) → add water to the residue → soak Stir → squeeze → filter to make juice for later use (2);

[0041] Two, the above-mentioned squeezing and filtering aqueous solution (1) and (2) are put together, and its water temperature is adjusted to the most suitable scope of following degrading biological enzyme;

[0042] 3. Biodegrade the above product with β-glucosidase that can cleave the glycosidic bond of anthocyanin, first stir at low speed, 50 rpm, and then let stand, so that the glycosidic bond in anthocyanin is degraded to obtain cationic anthocyanin Active aglycone;

[0043] The cracking and degradation time is 24 hours, the temperature is 50°C, and the pH is...

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Abstract

The invention relates to an extraction method of natural anthocyanidin active aglycone, and belongs to the field of bioengineering technology. The method comprises the following steps: classifying and grinding of the anthocyanidin-containing plants, water absorption, freezing, heating for unfreezing, squeezing and filtering to obtain juice, cracking and degradation of anthocyanidin with the bio-enzyme for cracking the glycosidic bond of anthocyanidin, a combination reaction with an anionic surfactant for precipitation or suspension, a replacement reaction with hydrochloric acid, separation of water insoluble matters, and freezing and drying of anthocyanidin aqueous solution. By adopting the technology, the method provided by the invention can obtain cationic anthocyanidin active aglycone with true content of 67% rather than anthocyanidin. The prepared cationic anthocyanidin does not need to be degraded by the intestinal microorganisms of human body and is directly absorbed and utilized by a human body or directly enters the blood of human body. The value in health care is much higher than that of anthocyanidin.

Description

Technical field: [0001] The invention relates to a method for extracting plant cationic anthocyanin active aglycone in the technical field of bioengineering, in particular to a method for extracting cationic anthocyanin aglycone. Background technique: [0002] With the improvement of people's living standards and the pursuit of natural organic substances that are non-toxic and harmless to natural plant sources, which are beneficial to protecting people's health, preventing and curing diseases, and because artificially synthesized anthocyanins have a greater impact on the human body Toxic and side effects have been banned by the World Health Organization; and anthocyanin industrial synthesis technology has large investment, complex and unstable technology, poor product quality, and cannot serve human beings. Therefore, efficient and non-toxic natural cationic anthocyanin active aglycones and their extraction methods have become emerging frontier research topics in the 21st ce...

Claims

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Application Information

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IPC IPC(8): C12P17/06
Inventor 耿兆翔
Owner 耿兆翔
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