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Flammulina velutipes spawn liquidation preparing method

A production method and technology for Flammulina velutipes, which are applied in the fields of botanical equipment and methods, fertilizer mixtures, horticulture, etc., can solve the problems of long growth cycle of solid strains, difficult transportation of liquid strains, and high investment costs, and achieve good performance characteristics and mushrooms. The effect of good uniformity and less liquefaction residue

Inactive Publication Date: 2014-06-04
TIANSHUI ZHONGXING BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Solid strains have disadvantages such as long growth cycle, inconsistent bacterial age, and poor quality, while liquid strains have disadvantages such as difficult transportation, storage, testing, miscellaneous removal and high investment costs, which have become the main factors restricting the development of industrial edible fungi; using solid The liquefaction technology of Flammulina velutipes strains, which is the direct expansion of strains to liquid strains, solves the shortcomings of liquid strains that are not easy to transport, store, detect, pick up impurities and high investment costs. It also has the advantages of neat fruiting of liquid strains, fast growth time, Advantages such as high output, but there is no record of the technology related to the liquefaction of Flammulina velutipes strains in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] A kind of liquefaction preparation method of Flammulina velutipes bacterial classification comprises the steps: 1) test tube culture slant medium is PDA enrichment medium: potato 200g, corn flour 20g, bran 50g, glucose 20g, KH 2 PO 4 1g, MgSO 4 1g, VB 1 10 pieces, agar 20g, water 1000ml; Insert the F8815 bacterial strain (Institute of Plant Protection, Chinese Academy of Agricultural Sciences) on the slope of the PDA-enriched medium, and place it at 25°C for constant temperature cultivation for 8-10 days; 2) The glass bottle culture medium is composed of raw materials, Water is composed at a weight ratio of 1:1.3, wherein the components of raw materials are: 45% pulp, 44% bagasse, 6.6% corn flour, 1.1% each of sugar, gypsum, lime and superphosphate, mixed and prepared Bottled at 1.5kg / cm 3 Sterilize under pressure and 121°C for 1.5 hours. When the temperature drops below 30°C, put the parent species formed after the strain test tube culture into the bottle, and cu...

Embodiment 2

[0010] A kind of liquefaction preparation method of Flammulina velutipes bacterial classification comprises the steps: 1) test tube culture slant medium is PDA enrichment medium: potato 200g, corn flour 20g, bran 50g, glucose 20g, KH 2 PO 4 1g, MgSO 4 1g, VB 1 10 pieces, agar 20g, water 1000ml; Insert the F8815 bacterial strain (Institute of Plant Protection, Chinese Academy of Agricultural Sciences) on the slope of the PDA-enriched medium, and place it at 25°C for constant temperature cultivation for 8-10 days; 2) The glass bottle culture medium is composed of raw materials, Water is composed by weight ratio of 1:1.2, wherein the components of said raw materials are by weight percentage: 50% of paper pulp, 40% of bagasse, 6% of corn flour, 1% each of sugar, gypsum, lime and superphosphate, mixed Bottled after preparation, at 1.5kg / cm 3 Sterilize under pressure and 121°C for 1.5 hours. When the temperature drops below 30°C, put the parent species formed after the strain ...

Embodiment 3

[0012] A kind of liquefaction preparation method of Flammulina velutipes bacterial classification comprises the steps: 1) test tube culture slant medium is PDA enrichment medium: potato 200g, corn flour 20g, bran 50g, glucose 20g, KH 2 PO 4 1g, MgSO 4 1g, VB 1 10 pieces, agar 20g, water 1000ml; Insert the F8815 bacterial strain (Institute of Plant Protection, Chinese Academy of Agricultural Sciences) on the slope of the PDA-enriched medium, and place it at 25°C for constant temperature cultivation for 8-10 days; 2) The glass bottle culture medium is composed of raw materials, Water is composed at a weight ratio of 1:1.25, wherein the components of raw materials are: 55% paper pulp, 36% bagasse, 5.4% corn flour, 0.9% each of sugar, gypsum, lime and superphosphate, mixed and prepared Bottled at 1.5kg / cm 3 Sterilize under pressure and 121°C for 1.5 hours. When the temperature drops below 30°C, put the parent species formed after the strain test tube culture into the bottle,...

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Abstract

The invention discloses a flammulina velutipes spawn liquidation preparing method which enables solid spawns to be directly prepared into liquid spawns in an expanding mode. The flammulina velutipes spawn liquidation preparing method includes the following steps: (1) preparing a culture medium with raw materials and water with the weight ratio ranging from 1:1.2 to 1:1.3, wherein the raw materials comprise, by weight, 45%-55% of paper pulp, 36%-44% of bagasse, 5.4%-6.6% of corn flour, 0.9%-1.1% of sugar, 0.9%-1.1% of gypsum, 0.9%-1.1% of lime and 0.9%-1.1% of calcium superphosphate; (2) cultivating stock cultures formed after tube culturing is carried out on bacterial strains, transplanting the stock cultures into the culture medium after hyphae are fully grown; (3) conducting liquidation to sample cultures, namely stock cultures, generated in the step (2), adding sterile water to conduct dilution according to the requirement that the weight ratio of spawn blocks to the sterile water ranges from 1:100 to 1:500, and evenly stirring the mixture.

Description

technical field [0001] The invention relates to a cultivation method of Flammulina velutipes strains, in particular to a liquefaction preparation method of Flammulina velutipes strains. Background technique [0002] Solid strains have disadvantages such as long growth cycle, inconsistent bacterial age, and poor quality, while liquid strains have disadvantages such as difficult transportation, storage, testing, miscellaneous removal and high investment costs, which have become the main factors restricting the development of industrial edible fungi; using solid The liquefaction technology of Flammulina velutipes strains, which is the direct expansion of strains to liquid strains, solves the shortcomings of liquid strains that are not easy to transport, store, detect, pick up impurities and high investment costs. It also has the advantages of neat fruiting of liquid strains, fast growth time, The advantages such as high output, but there is no record of the technology related t...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
Inventor 陶军
Owner TIANSHUI ZHONGXING BIO TECH
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