New mutation sites associated with streptomycin resistance in Mycobacterium tuberculosis and their applications

Abstract

The invention discloses a new mutation site related to streptomycin resistance of Mycobacterium tuberculosis and its application. The new mutation site is located at the 1338631st position of the standard strain H37Rv genome, that is, the 47th base of the intergenic region Rv1194c‑Rv1195 , the point mutation that occurs is the mutation of base C to A. The new mutation site found in the present invention can be used as a detection target in the detection of streptomycin resistance of Mycobacterium tuberculosis, improve the molecular detection level of streptomycin resistance of Mycobacterium tuberculosis, shorten the diagnosis time of patients, save Patient treatment time and cost. The invention shows that the tuberculosis drug-resistant mutation site may also occur in the non-coding region, suggesting that the base mutation in the intergenic region may also be related to drug resistance, and there are also drug-resistant biomarkers. The invention provides a new idea for finding new and more effective tuberculosis drug resistance molecular markers.

Description

technical field [0001] The invention relates to high-throughput screening and verification of drug-resistant base mutation sites of Mycobacterium tuberculosis, in particular to the screening and verification of drug-resistant mutation sites of Mycobacterium tuberculosis streptomycin. Background technique [0002] Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis infection. Mycobacterium tuberculosis may invade various organs of the human body, but mainly invades the lungs, which is called pulmonary tuberculosis. By the late 1980s, due to factors such as poverty, AIDS, population migration, and drug resistance, the incidence and mortality of tuberculosis rose rapidly around the world, becoming the most serious infectious disease in the world alongside AIDS. The drug resistance of Mycobacterium tuberculosis is serious, and the drug resistance rate is as high as 46%, which poses a severe challenge to the prevention and treatment of tuberculosis....

AI Technical Summary

Problems solved by technology

These methods mainly use genetic rPS The A→G mutation standard of the 43rd base is used to detect whether the same mutation occurs in Mycobacterium tuberculosis in the sample, so as to quickly determine whether Mycobacterium tuberculosis is resistant to streptomycin, but there are still many streptomycin-resistant Mycobacterium tuberculosis cannot be detected, false negative

[0005] The high drug resistance rate of tuberculosis is an important reason for the difficulty of controlling the tuberculosis epidemic. However, the traditional drug susceptibility test of Mycobacterium tuberculosis takes a long time, affects the diagnosis and treatment of patients, and increases the chance of drug-resistant strains spreading. Therefore, the rapid identification of tuberculosis by molecular biological methods can be effectively improved. The detection rate and accuracy of mycobacterial resistance are of great significance

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