Chinese herbal medicine granular preparation used for aquatic product disinfection and preparing method thereof
A technology for disinfection and preparation, applied in the directions of disinfectants, botanical equipment and methods, chemicals for biological control, etc., can solve the problems of deterioration of pond bottom and incomplete disinfection of pond bottom.
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Embodiment 1
[0018] 1. Screen out Chinese herbal medicines that can inhibit Aeromonas hydrophila and Vibrio parahaemolyticus, such as rhubarb, cork, coptis, clove, mint, citronella, and thyme.
[0019] 2. Carry out the bacteriostasis test of Aeromonas hydrophila and Vibrio parahaemolyticus through the orthogonal test on the various medicinal materials screened out, and carry out significance analysis to obtain the appropriate formula ratio.
[0020] 3. Weigh by weight:
[0021] Rhubarb 300g
[0022] Cork 200g
[0023] Coptis 100g
[0024] Mix the medicinal materials evenly and put them into the traditional Chinese medicine extraction tank. For the first extraction: add 6L of water, and the extraction temperature is 100°C. When the temperature rises to 100°C, start timing. The constant temperature time is 1.5h. Filter through 4 layers of gauze and pour into the stock solution in the can. The second extraction: Add 4.8L of water, the extraction temperature is 100°C, start timing when it ...
Embodiment 2
[0033] Embodiment 2: the antibacterial effect investigation of Chinese herbal medicine granule of the present invention
[0034] 1 Test purpose: To detect the bacteriostatic effect of Chinese herbal medicine granules of the present invention on Aeromonas hydrophila and Vibrio parahaemolyticus.
[0035] 2. Instruments and medicines
[0036] 2.1 Test equipment: clean bench, autoclave, artificial climate box, electronic analytical balance
[0037] 2.2 Test strains: Aeromonas hydrophila, Vibrio parahaemolyticus. purchased from the General Microbiology Center of China Committee for Culture Collection of Microorganisms.
[0038] 2.3 Reagents: sodium chloride (analytical grade), beef extract (biochemical grade), peptone (biochemical grade), Chinese herbal medicine granules of the present invention are provided by Beijing Shengtaier Biotechnology Co., Ltd.
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