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Ruminant micro-ecological functional feed prepared through compound fermentative bacteria solution and preparation method of feed

The technology of compound fermentation bacteria and functional feed is applied in the field of ruminant micro-ecological functional feed and production of compound fermentation bacteria liquid, which can solve the problems of large equipment investment and achieve the effect of simple and easy process.

Inactive Publication Date: 2014-05-21
SOUTHWEST UNIVERSITY FOR NATIONALITIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yan Qitao et al. (2000) used photosynthetic bacteria (PSB) to ferment corn yellow pulp water to produce PSB bacteria slime (Run Qitao et al., Application Prospects of PSB Resource Development of Corn Starch Yellow Syrup Water), Journal of Jilin Grain College, 2000 Phase 4), this technology needs to separate the fermentation liquid from the bacterial liquid to make the liquid discharge up to the standard, but it also requires a large investment in equipment

Method used

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  • Ruminant micro-ecological functional feed prepared through compound fermentative bacteria solution and preparation method of feed
  • Ruminant micro-ecological functional feed prepared through compound fermentative bacteria solution and preparation method of feed
  • Ruminant micro-ecological functional feed prepared through compound fermentative bacteria solution and preparation method of feed

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Experimental program
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Effect test

Embodiment 1

[0060] Embodiment 1, the screening of lactic acid bacteria and performance measurement

[0061] 1.1. Screening yak gastrointestinal lactic acid bacteria to obtain LAg12-7 Lactobacillus acidophilus

[0062] MRS medium: peptone 10.00g, yeast extract 5.00g, beef extract 10.00g, glucose 20.00g, dipotassium hydrogen phosphate 2.00g, triammonium citrate 1.00g, sodium acetate 5.00g, magnesium sulfate 0.80g, manganese sulfate 0.25g , Tween 80 1ml, dilute to 1000ml with distilled water, pH 6.2~6.4, sterilize at 115°C for 30min.

[0063] Using conventional microbiological methods, 108 strains of lactic acid bacteria were isolated from yak gastrointestinal mucosa, and a strain with excellent performance was screened out through acid production, acid resistance and salt resistance experiments. After physiological and biochemical identification and 16SrDNA molecular identification, the strain was determined to be Lactobacillus acidophilus and named Lactobacillus acidophilus LAg12-7. The s...

Embodiment 2

[0083] Embodiment 2, the method for preparing the composite fermented bacterial agent that ruminant micro-ecological function feed is used

[0084] The composition and weight ratio of the composite fermentation agent are: LAg12-7 Lactobacillus acidophilus freeze-dried powder 30.0%, Bacillus subtilis freeze-dried powder 50.0%, Saccharomyces cerevisiae freeze-dried powder 20.0%. The composite fermented bacterial agent for ruminant micro-ecological function feed is prepared by mixing LAg12-7 Lactobacillus acidophilus freeze-dried powder, Bacillus subtilis freeze-dried powder and brewer's yeast freeze-dried powder evenly under normal temperature conditions.

[0085]

Embodiment 3

[0086] Embodiment 3, the method for preparing the composite fermented bacterial agent that ruminant micro-ecological function feed is used

[0087] The composition and weight ratio of the composite fermentation agent are: LAg12-7 Lactobacillus acidophilus lyophilized powder 60.0%, Bacillus subtilis lyophilized powder 20.0%, Saccharomyces cerevisiae lyophilized powder 20.0%. The composite fermented bacterial agent for ruminant micro-ecological function feed is prepared by mixing LAg12-7 Lactobacillus acidophilus freeze-dried powder, Bacillus subtilis freeze-dried powder and brewer's yeast freeze-dried powder evenly under normal temperature conditions.

[0088]

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Abstract

The invention relates to a ruminant micro-ecological functional feed prepared through a compound fermentative bacteria solution and a preparation method of the feed, and belongs to the technical field of application of biological bacteria in feeds. The invention relates to a preferred LAg12-7 lactobacillus acidophilus, and particularly relates to a method for preparing the ruminant micro-ecological functional feed after food industry wastes, feed raw materials and auxiliary materials are fermented by using the compound fermentative bacteria solution which contains the LAg12-7 lactobacillus acidophilus. The preferred LAg12-7 lactobacillus acidophilus disclosed by the invention has a good acid-producing property, an acid-resisting property and a bacteria-inhibiting property, achieves the very good compatibility with bacillus subtilis and saccharomyces cerevisiae, fast reduces the pH value of a fermentive feed below 4.0, fast inhibits harmful microbes and enables that the quality of the fermentive feed can be guaranteed for a long time under the condition that beneficial bacteria are in a preponderant state all the time. The ruminant micro-ecological functional feed prepared by adopting the method disclosed by the invention has the advantages of simplicity and easiness for operation of a process, low production cost, convenience for feeding, long guarantee period, enhancement of the digestibility of daily ration cellulose and the like, and good economic benefits.

Description

technical field [0001] (This application is a divisional application, and the original application is Chinese patent application 201210399518.8) The present invention belongs to the technical field of the application of microbial beneficial bacteria in feed, and relates to a preferred strain of Lactobacillus acidophilus, especially to a compound fermentation containing the Lactobacillus acidophilus The microbial agent is used for the method of preparing the micro-ecological functional feed for ruminants by using food industry waste as feed raw material and auxiliary material after mixing and fermenting. [0002] Background technique [0003] Micro-ecological functional feed is a feed obtained by fermenting relevant feed materials with a variety of beneficial microorganisms. The microorganisms and metabolites in it are consistent with the natural micro-ecological system in related animals and are nutritionally complementary. The micro-ecological functional feed produced by m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/16A23K1/14A23K1/175
CPCY02P60/87
Inventor 郭春华彭忠利罗璠柏雪王永玲
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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