Apparatus and method for gradient temperature control

A gradient temperature and constant temperature control technology, which is applied in the direction of temperature control using electric means, biochemical equipment and methods, biochemical cleaning devices, etc., can solve the problems of overshoot, shortening the service life, affecting the heating and cooling rate of the sample module, etc. , to achieve the effect of flexible temperature control, extended service life and scalability

Active Publication Date: 2016-01-13
ZHEJIANG UNIV
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  • Abstract
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Problems solved by technology

The disadvantages of this method are: (1) use the same temperature control module to achieve heating and cooling in a short period of time, and sudden changes in temperature can easily damage the temperature control module and shorten its service life; (2) During the temperature control process of the sample module (that is, the controlled target, the same below), the temperature control module itself has a process of heating and cooling, which affects the rate of heating and cooling of the sample module. Temperature has overshoot phenomenon, which requires complex control algorithm to eliminate or weaken this phenomenon

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  • Apparatus and method for gradient temperature control

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Embodiment 1

[0027] see Figure 1-4 , a translational device suitable for gradient PCR reactions based on the principle of time-division multiplexing of temperature control modules, consisting of a first temperature control module 1, a second temperature control module 2, a control module 3, a transmission device 4, and a controlled target 5 Composed of a detection module 6, the first temperature control module 1 and the second temperature control module 2 are connected to the transmission device 4, and the transmission device 4 controls different temperature control modules to move or rotate and physically contact the controlled target 5. The control module 3 and the The transmission device 4 is connected, and the control module 3 outputs control signals to control the transmission device 4 to generate corresponding actions. The signal controls the temperature control module to realize temperature changes. The detection module 6 is connected to the controlled target 5. The detection modul...

Embodiment 2

[0033] see figure 1 and figure 2 , is a schematic structural view of two PCR thermal cyclers based on the present invention, because the transmission device 4 in the two examples realizes the controlled target 5 and the temperature control module (the first temperature control module 1, the second temperature control module 2 ) linear motion in physical contact, so they are explained together. The difference between the two is figure 1 Using translational transmission, figure 2 Use the longitudinal transmission device, which can be selected according to actual needs. The two PCR thermal cyclers mainly include a control module 3 , a first temperature control module 1 , a second temperature control module 2 , a transmission device 4 , a controlled target 5 and a detection module 6 .

[0034] The temperature control modules (the first temperature control module 1 and the second temperature control module 2 ) include a heating module, a cooling module, a heat dissipation mod...

Embodiment 3

[0044] see image 3 , Figure 4 , is a structural schematic diagram of a disc-type PCR thermal cycler based on the present invention, the corresponding device uses a rotating device 11 to realize rotational positioning, and realizes the controlled target 5 and the temperature control module (the first temperature control module 1, the second temperature control module physical contact with the control module 2). It should be noted that the shapes of the controlled target 5 and the temperature control module suitable for the rotating device 11 are not limited to the shape of a disc, but can also be fan-shaped, diamond-shaped, square, polygonal, and irregular polygonal, etc. Figure 4 It is a top view structure schematic diagram of a supporting sample loading disc. Figure 7 (Patent: CN102277294B figure 2 ) is a schematic diagram of an integrated flow chip suitable for performing digital PCR reactions in the device of the present invention. The integrated flow path chip mod...

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Abstract

The invention provides a device applied to gradient temperature control, which is composed of a first temperature control module, a second temperature control module, a control module, a transmission device or a rotating device, a controlled target and a detection module. The method and device of the present invention have scalability, different temperature control modules can be used alternately at different time points, and the temperature control module in the non-working state can use the idle time to achieve the next target temperature value without sudden changes, prolonging its Service life; the temperature control module in the working state does not have a process of heating and cooling, which increases the rate of heating and cooling of the sample module to avoid overshooting, and does not require complex control algorithms. The temperature control is more flexible, it can shorten the effective temperature control time of the controlled target, increase the temperature change rate of the controlled target, and can be applied in gradient temperature control. Using a small number of temperature control modules, using the "time-division multiplexing" idea to use different temperature control modules to control the controlled target temperature in different time periods, so as to achieve multiple target gradient temperatures.

Description

technical field [0001] The invention belongs to the field of temperature control, and relates to a gradient temperature control device and method, which can be applied to occasions where a controlled target needs to realize multiple target temperature values. For example, the polymerase chain reaction (PCR) in the fields of biology and medicine requires the target sample to achieve three processes of high temperature denaturation, low temperature annealing, and medium temperature extension, corresponding to three different gradient temperatures, and these three different gradient temperatures are required to achieve Multiple thermocycling to complete the PCR amplification reaction. Background technique [0002] Some related technologies in many fields such as biology, chemistry, medicine, industry, etc. need to use thermal cycle to realize. [0003] Take the "polymerase chain reaction" (PCR), which is common in the field of biology, as an example. In order to realize the P...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G05D23/30C12M1/38
Inventor 牟颖于丙文朱强远宋博凡金钦汉金伟
Owner ZHEJIANG UNIV
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