Apparatus and method for gradient temperature control

Abstract

The invention provides a device applied to gradient temperature control, which is composed of a first temperature control module, a second temperature control module, a control module, a transmission device or a rotating device, a controlled target and a detection module. The method and device of the present invention have scalability, different temperature control modules can be used alternately at different time points, and the temperature control module in the non-working state can use the idle time to achieve the next target temperature value without sudden changes, prolonging its Service life; the temperature control module in the working state does not have a process of heating and cooling, which increases the rate of heating and cooling of the sample module to avoid overshooting, and does not require complex control algorithms. The temperature control is more flexible, it can shorten the effective temperature control time of the controlled target, increase the temperature change rate of the controlled target, and can be applied in gradient temperature control. Using a small number of temperature control modules, using the "time-division multiplexing" idea to use different temperature control modules to control the controlled target temperature in different time periods, so as to achieve multiple target gradient temperatures.

Description

technical field [0001] The invention belongs to the field of temperature control, and relates to a gradient temperature control device and method, which can be applied to occasions where a controlled target needs to realize multiple target temperature values. For example, the polymerase chain reaction (PCR) in the fields of biology and medicine requires the target sample to achieve three processes of high temperature denaturation, low temperature annealing, and medium temperature extension, corresponding to three different gradient temperatures, and these three different gradient temperatures are required to achieve Multiple thermocycling to complete the PCR amplification reaction. Background technique [0002] Some related technologies in many fields such as biology, chemistry, medicine, industry, etc. need to use thermal cycle to realize. [0003] Take the "polymerase chain reaction" (PCR), which is common in the field of biology, as an example. In order to realize the P...

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Problems solved by technology

The disadvantages of this method are: (1) use the same temperature control module to achieve heating and cooling in a short period of time, and sudden changes in temperature can easily damage the temperature control module and shorten its service life; (2) During the temperature control process of the sample module (that is, the controlled target, the same below), the temperature control module itself has a process of heating and cooling, which affects the rate of heating and cooling of the sample module. Temperature has overshoot phenomenon, which requires complex control algorithm to eliminate or weaken this phenomenon

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