Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting propranlolum rapidly and flexibly based on nanogold chemiluminiscence

A chemiluminescence and sensitive detection technology, which is applied in chemiluminescence/bioluminescence, analysis through chemical reaction of materials, instruments, etc., can solve the problems of unsatisfactory sensitivity, large and expensive, high requirements of analysts, etc. Automated analysis operation, shortened analysis and test time, and improved sensitivity

Inactive Publication Date: 2014-04-30
FUJIAN UNIV OF TECH
View PDF4 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention solves the limitations of the traditional drug determination method: using large and expensive instruments, the analysis cost is high; the sensitivity cannot meet the needs of various aspects; the operation is troublesome and the requirements for analysts are high; the analysis and determination time is long

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting propranlolum rapidly and flexibly based on nanogold chemiluminiscence
  • Method for detecting propranlolum rapidly and flexibly based on nanogold chemiluminiscence
  • Method for detecting propranlolum rapidly and flexibly based on nanogold chemiluminiscence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0026] The main equipment and reagents used:

[0027] IFFL-D flow injection chemiluminescence analyzer and its supporting equipment (Xi'an Ruimai Analytical Instrument Co., Ltd.), TU-1901 ultraviolet-visible spectrophotometer (Beijing General Instrument Co., Ltd.), transmission electron microscope (Hitachi, Specification model: H-600).

[0028] Chlorauric acid (HAuCl 4 4H 2 O, AR, Au content > 47.8%, Sinopharm Chemical Reagent Co., Ltd.); the chloroauric acid stock solution was prepared by dissolving the chloroauric acid solid in secondary deionized water, and stored in a refrigerator at 4 °C. Dissolve 4.43 g of luminol solid powder in 20 mL of 0.10 M NaOH solution and dilute to 1 L to prepare 2.5 × 10 2 M Luminol stock soluti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for detecting propranlolum rapidly and flexibly based on nanogold chemiluminiscence. The method comprises the following steps: 1) preparing a nanogold solution by adopting a sodium borohydride reduction method; 2) analyzing and determining the propranlolum by adopting a flow injection chemiluminiscence analysis system, wherein the particular flow path is as follows: firstly, mixing a luminal solution and H2O2, introducing the nanogold and propranlolum solution into an analysis system by taking secondary deionized water as carrying flow, introducing the luminal solution, the H2O2 and the secondary deionized water into a circulation tank by a peristaltic pump, injecting the mixed solution of nanogold and the propranlolum by a sample injection valve; detecting chemiluminiscence signals by a photomultiplier close to a flow injection chemiluminiscence tank; and then inputting the chemiluminiscence signals into a computer, and recording and collecting data. The detection limit can be up to 8.2*10<-12>g / mL, and the sensitivity is improved by one order compared with that of a conventional chemiluminiscence analysis method, and the analysis and testing time is shorted within 10 minutes.

Description

technical field [0001] The invention relates to the technical field of drug detection, in particular, the invention relates to a method for rapid and sensitive detection of propranolol based on nano-gold chemiluminescence. Background technique [0002] Propranolol is a β-adrenoceptor blocker, the chemical name is 1-isopropylamino-3-(1-naphthyloxy)-2-propanol hydrochloride, widely used in the treatment of cardiovascular diseases, Drugs belonging to the antihypertensive class. In recent years the drug has been used as a stimulant to improve athletes' performance in competitions, and the International Olympic Committee has listed it as a banned drug. Therefore, its sensitive determination is very important. In addition, drug sampling inspection is an important means of drug supervision, and the country spends a lot of money and time on sampling inspection every year. At present, the grassroots drug supervision departments in my country have a heavy workload and a small numbe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N35/08
Inventor 齐莹莹修福荣
Owner FUJIAN UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com