Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extraction of kallikrein by acetic acid

A technology of kallikrein and acetic acid, applied in peptidase, biochemical equipment and methods, enzymes, etc., can solve the problems of shortening the life of ion exchange media, affecting product yield and specific activity, prolonging production cycle, etc.

Active Publication Date: 2014-04-23
QINGDAO KANGYUAN PHARMA
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In actual large-scale production, the raw materials contain impurities. If the raw materials or crude products are not pretreated before adopting these high-end chromatographic technologies, the life of the ion exchange medium will be shortened due to the excessive content of impurities in the raw materials, and the Resin adsorption efficiency, and there will be tailing phenomenon in the elution, prolong the production cycle, affect the product yield and specific activity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] After said extracting and precipitating porcine pancreas to make acetone powder by using porcine pancreas as raw material, traditional protein purification techniques such as acetic acid extraction, acetone precipitation, ether degreasing and dehydration are used flexibly to prepare kallikrein, which includes the following steps:

[0013] 1. Acetic acid extraction: After making 10Kg of pig pancreas into acetone powder, add 20 times the amount of 0.02mol / L acetic acid solution, stir and extract at 10°C for 12 hours, centrifuge to obtain the supernatant; add 10 times the amount of 0.02mol to the residue / L acetic acid solution was stirred and extracted again for 6 hours, centrifuged, and 29.8 L of the two supernatants were combined.

[0014] 2. Acetone precipitation: collect the supernatant, add acetone at 5°C to make the concentration of the solution reach 33%, stir well, filter to obtain the filtrate; add acetone at 5°C to the filtrate until the concentration of the solu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extraction of kallikrein by acetic acid. The technical proposal of the method is as follows: taking porcine pancreas as a raw material for extraction and precipitation of the porcine pancreas to obtain acetone power, then flexibly using traditional protein purification technologies such as extraction with the acetic acid, precipitating with acetone, degreasing and dehydrating with diethyl ether and the like to prepare the kallikrein. The method has the advantages of simple operation, short production cycle and low production cost, and is very suitable for large scale industrial production.

Description

technical field [0001] The invention relates to the field of biotechnology. Specifically, kallikrein is prepared by extracting porcine pancreas as a raw material, precipitating porcine pancreas to make acetone powder, and flexibly using traditional protein purification techniques such as acetic acid extraction, acetone precipitation, ether degreasing and dehydration. Background technique [0002] Kallikrein (kallikein) is a natural enzyme drug isolated and purified from porcine pancreas. It is also called kalikrein clinically. It has the effect of stretching capillaries and arterioles. It is often used clinically to treat hyperlipidemia. Prevention and treatment of fatty liver, atherosclerosis, hypertension, angina pectoris and other diseases, its development has important medical and social value. In my country, there is still a lot of room for development in the production of kallikrein. [0003] The traditional process of purifying kallikrein is complicated and costly; i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N9/64
CPCC12N9/6445C12Y304/21008
Inventor 刘乃山林晓磊李静洁刘君
Owner QINGDAO KANGYUAN PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com