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Flaxbush tissue culture method

A tissue culture and tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of small number of introduced seeds, limited seedlings, large market demand, etc., to improve the uniformity and good quality of seedlings. Maternal traits, the effect of increasing the speed of reproduction

Active Publication Date: 2014-04-23
上海上房园艺有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the number of species introduced from abroad is small, its propagation is slow, the market demand is large, and the supply of seedlings is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for tissue culture of New Zealand hemp, the method comprising the following steps:

[0035] (1) The preparation of the culture medium, including the components and contents of the culture medium at each stage of tissue culture:

[0036] 1) Bud induction medium: MS+6-BA5mg / L+NAA5mg / L;

[0037] 2) Adventitious bud proliferation medium: MS+6-BA3mg / L+NAA0.3mg / L;

[0038] 3) Strong seedling medium: MS+6-BA0.1mg / L+NAA0.1mg / L;

[0039] 4) Rooting medium: MS+NAA0.1mg / L;

[0040] For the pH5.8 of the above-mentioned medium, add 30g / .L sucrose, 6g / L agar, culture temperature is (25±1)℃, light is about 80μmol ms:

[0041] (2) Tissue culture of New Zealand hemp

[0042] 1) Obtaining sterile materials

[0043] Dig out the small buds at the base of the New Zealand hemp plant, wash them with tap water for 2 hours, put them on an ultra-clean workbench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for 15 minutes, wash them with sterile water 5-6 t...

Embodiment 2

[0053] A method for tissue culture of New Zealand hemp, the method comprising the following steps:

[0054] (1) The preparation of the culture medium, including the components and contents of the culture medium at each stage of tissue culture:

[0055] 1) Bud induction medium: MS+6-BA1.0mg / L+NAA0.1mg / L;

[0056] 2) Adventitious bud proliferation medium: MS+6-BA1mg / L+NAA0.1mg / L;

[0057] 3) Strong seedling medium: MS+6-BA0.5mg / L+NAA0.1mg / L;

[0058] 4) Rooting medium: MS+NAA0.05mg / L;

[0059] For the pH5.8 of the above-mentioned medium, add 30g / .L sucrose, 6g / L agar, culture temperature is (25±1)℃, light is about 70μmol ms:

[0060] (2) Tissue culture of New Zealand hemp

[0061] 1) Obtaining sterile materials

[0062] Dig out the small buds at the base of the New Zealand hemp plant, wash them with tap water for 2 hours, put them on an ultra-clean workbench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for 15 minutes, wash them with sterile water ...

Embodiment 3

[0072] A method for tissue culture of New Zealand hemp, the method comprising the following steps:

[0073] (1) The preparation of the culture medium, including the components and contents of the culture medium at each stage of tissue culture:

[0074] 1) Bud induction medium: MS+6-BA3.0mg / L+NAA0.3mg / L;

[0075] 2) Adventitious bud proliferation medium: MS+6-BA2mg / L+NAA0.2mg / L;

[0076] 3) Strong seedling medium: MS+6-BA2mg / L+NAA0.2mg / L;

[0077] 4) Rooting medium: MS+NAA0.2mg / L;

[0078] For the pH5.8 of the above-mentioned medium, add 30g / .L sucrose, 6g / L agar, cultivate at (25±1)℃, and light about 90μmol ms:

[0079] (2) Tissue culture of New Zealand hemp

[0080] 1) Obtaining sterile materials

[0081] Dig out the small buds at the base of the New Zealand hemp plant, wash them with tap water for 2 hours, put them on an ultra-clean workbench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercury liter for 15 minutes, wash them with sterile water 5-6 times, a...

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PUM

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Abstract

The invention relates to a flaxbush tissue culture method. The method comprises the following steps: (1) preparing a culture medium comprising components and content of the culture medium in each stage during tissue culture, 1) a bud induction culture medium: MS+6-BA(1.0-5.0)mg / L+NAA(0.1-0.5)mg / L; 2) an adventitious bud proliferation culture medium: MS+6-BA(1.0-3.0)mg / L+NAA(0.1-0.3)mg / L; 3) a strong seedling culture medium: MS+6-BA(0.5-2.0)mg / L+NAA(0.1-0.2)mg / L; 4) a rooting culture medium: MS+NAA(0.05-0.2)mg / L, wherein the ph values of the culture mediums under various conditions is 5.8; adding 30g / L of cane sugar, 6g / L of agar, the culture temperature is 25+ / -1 DEG C, and the illumination is about 80 micromoles. Compared with the prior art, the flaxbush tissue culture method has the advantages that the breeding speed and the seedling uniformity can be greatly improved, and the original female parent characters are well kept.

Description

technical field [0001] The invention relates to plant cultivation, in particular to a tissue culture method of New Zealand hemp. Background technique [0002] New Zealand hemp is a shrub plant of the Agaveaceae New Zealand genus Hemp, which is a perennial evergreen herb. New Zealand hemp has no central erect stem, but has short, fleshy, creeping, forked running stems, about 5 cm in diameter. A group of strong, sword-shaped leaves emerge from the walking stems, slightly fanned in growth, strong and thick leathery, basal. The panicle is born on the leafless flower stem, the base of the flower is cylindrical, the corolla is dark red, and it blooms in summer. Capsule erect, with three edges. It likes a warm and sunny environment. It is suitable for growth in deep, humus-rich sandy loam. It is not cold-resistant. It is more suitable for garden cultivation or flower border configuration. It is an excellent foliage plant with beautiful plant shape, gorgeous and elegant colors, a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 黄建荣张浪陈建华沈勤
Owner 上海上房园艺有限公司
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