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Coli group and colibacillus testing medium

A technology of Escherichia coli and coliform bacteria, applied in the field of culture medium, can solve the problems of high cost and unfavorable popularization and use, and achieve the effect of low cost and favorable popularization and use

Inactive Publication Date: 2014-04-16
STONE LAKE PHARMA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the reagents for simultaneous detection of coliforms and E. coli mainly use 5-bromo-4-chloro-3-indole-galactoside (X-Gal) and 5-bromo-4-chloro-3-indole-β-D -Glucuronide (X-Gluc) two enzyme substrates, the cost is too high, not conducive to popular use

Method used

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Embodiment Construction

[0010] Coliform and E. coli detection medium, consisting of 20g peptone, 5g lactose, 2g bile salt, 1.5g potassium dihydrogen phosphate, 4g potassium dihydrogen phosphate, 5g sodium chloride, 5-bromo-4-chloro-3- 0.1 g of indolyl galactoside, 0.1 g of 4-methylfanone glucuronide, 1 g of aspartic acid, 0.5 g of casamino acids, 1 g of yeast extract and 20 g of agar are dissolved in 1000 ml of deionized water.

[0011] Further, the 5-bromo-4-chloro-3-indolyl galactoside is a β-galactosidase substrate.

[0012] Further, the 4-methylfanone glucuronide is a substrate of β-D-galactosidase.

[0013] Principle of the present invention is:

[0014] 1. It is composed of peptone, lactose, bile salt and other basic nutrients required by coliform bacteria and coliform selective substances, and added β-galactosidase substrate 5-bromo-4-chloro-3-indole X-galactoside (X-GAL) makes the color of coliform bacteria, and the addition of β-D-galactosidase substrate 4-methylfanone glucuronide (MUG) ma...

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Abstract

The invention discloses a coli group and colibacillus testing medium which is prepared by dissolving 20 grams of peptone, 5 grams of lactose, 2 grams of bile salt, 1.5 grams of monopotassium phosphate, 4 grams of monopotassium phosphate, 5 grams of sodium chloride, 0.1 gram of 5-bromo-4-chloro-3-indole galactoside, 0.1gram of 4- methylumbelliferone glucuronide, 1 gram of aspartic acid, 0.5 gram of casamino acid, 1 gram of yeast extracts and 20 grams of agar in 1000ml of deionized water, and the coli group and colibacillus testing medium is not only used as a plate medium, can also be used as an analoids form, and is low in cost and convenient to popularize and use.

Description

technical field [0001] The invention relates to a culture medium, in particular to a detection culture medium for coliforms and Escherichia coli. Background technique [0002] Coliform bacteria are widely distributed and widely exist in feces of warm-blooded animals and in nature. Investigations and studies have shown that coliform bacteria mostly exist in the feces of warm-blooded animals, places where humans are frequently active, and places with feces pollution. The pollution of human and animal feces to the external environment is the main reason for the existence of coliforms in nature. Most of the feces are dominated by typical Escherichia coli, while other types of coliforms are more common in the external environment. Coliform bacteria is one of the important indicators to evaluate the quality of food hygiene. It has been widely used in food hygiene work at home and abroad, and Escherichia coli is an important indicator to evaluate food hygiene. At present, the rea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12R1/19
Inventor 金京勋肖香吴小微
Owner STONE LAKE PHARMA TECH
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