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Bovine semen freezing dilute solution containing trace amount of rare-earth and application thereof

A technology for freezing diluent and diluent, which is applied in the field of diluent to achieve the effects of simple preparation, reduction of deformity rate, and acceleration of repair

Inactive Publication Date: 2014-04-09
SHANDONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching, it contains trace rare earth CeCl 3 The frozen dilution of bovine semen and its application have not been reported yet

Method used

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  • Bovine semen freezing dilute solution containing trace amount of rare-earth and application thereof
  • Bovine semen freezing dilute solution containing trace amount of rare-earth and application thereof
  • Bovine semen freezing dilute solution containing trace amount of rare-earth and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Embodiment 1, the preparation of bovine semen freezing diluent

[0020] With different concentrations of CeCl 3 The preparation method of the frozen diluent: add a small amount of rare earth CeCl to the Casu diluent 3 , so that CeCl 3 The final concentrations were 0.03μmol / L, 0.1μmol / L, 0.3μmol / L, 1μmol / L, 3μmol / L.

Embodiment 2

[0021] Embodiment 2, the acquisition and cryopreservation of bovine semen

[0022] 1. Obtaining bovine semen

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PUM

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Abstract

The invention discloses a bovine semen freezing dilute solution which contains a trace amount of rare-earth. The component of the bovine semen freezing dilute solution takes a commercially available cassou dilute solution as an application matrix and also contains the trace amount of rare-earth CeCl3 with the final concentration of 0.03-3 micromoles / liter. The invention discloses application of the bovine semen freezing dilute solution to cryopreservation of bovine semen. An experiment proves that the bovine semen freezing dilute solution disclosed by the invention accumulatively enhances five indexes for evaluating semen quality by 29% compared with a traditional freezing dilute solution; the bovine semen freezing dilute solution disclosed by the invention is easy to prepare, outstandingly superior to the traditional freezing dilute solution in total effect and worthy of popularization and application, and the source of the CeCl3 rare-earth guaranteed.

Description

technical field [0001] The invention relates to a diluent used for cryopreservation and its application, in particular to a bovine semen freezing diluent containing a trace of rare earth and its application. Background technique [0002] In animal husbandry, the application of livestock semen cryopreservation technology provides an important and effective technical basis for the development of highly intensive modern animal husbandry. Semen freezing technology has many advantages: (1) It can reduce the breeding amount of male animals and save costs; (2) Artificial insemination with frozen semen is not restricted by region and time, which can effectively improve the utilization rate of breeding males and reduce the spread of diseases; ( 3) The organic combination of semen freezing technology with artificial insemination, in vitro fertilization, gender control and other modern biotechnological means will play a huge role in improving livestock breeds, improving livestock produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 孔维华仲跻峰何乃森王长法张尚立彭正华
Owner SHANDONG UNIV
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