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Bacillus amyloliquefaciens, microbial inoculant and application thereof

A technology for dissolving starch spores and microbial inoculants, applied in the directions of microorganisms, biocides, microorganism-based methods, etc., to achieve the effects of increasing photosynthetic area, promoting nutrient production and reproductive growth, and improving peanut yield

Active Publication Date: 2014-04-02
BIOLOGY INST OF HEBEI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the biological control of peanut leaf spot. The purpose of the present invention is to screen out a microbial strain that can efficiently antagonize the pathogenic bacteria of peanut leaf spot, and form a microbial agent to reduce peanut leaf spot by biological control. Harm caused by spot disease to peanut production

Method used

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  • Bacillus amyloliquefaciens, microbial inoculant and application thereof
  • Bacillus amyloliquefaciens, microbial inoculant and application thereof
  • Bacillus amyloliquefaciens, microbial inoculant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Isolation and Screening of Bacteria with Antagonism to Peanut Leaf Spot

[0041] Soil samples from different ecological environments were collected and bacteria were isolated in the laboratory. After repeated isolation and purification on PB medium, after obtaining pure culture bacteria, Cercospora coccoides was used as the indicator bacteria (such as figure 2 Shown), the strains with high antagonistic performance were screened by the confrontation test method.

[0042] Specific steps: the above-mentioned soil samples were spread in PB medium after gradient dilution, and cultured in a constant temperature incubator at 28°C. The above-mentioned medium was prepared according to the records in the "Microbial Culture Medium Manual". The formula of the medium is: 5g of beef extract, 10g of peptone, 5g of sodium chloride, 15g of agar, 1000ml of tap water, pH7.2~7.4, sterilized at 121°C for 20min use. After colonies grow on the plate, single colonies of different ...

Embodiment 2

[0044] The antibacterial spectrum determination of embodiment 2 BA-KA1

[0045] BA- The antibacterial spectrum of KA1, BA-KA1 has a strong antagonistic effect on 9 kinds of pathogenic fungi, and the antagonistic effect on all pathogenic bacteria is more than 40%, among which the inhibitory effect on tomato early blight is the strongest, which is 74.3% .

[0046] Table 1 Inhibitory effect of Bacillus amyloliquefaciens BA-KA1 on different pathogenic fungi

[0047]

Embodiment 3

[0048] Example 3 Identification of antagonistic strains

[0049] According to the experimental content and experimental methods recorded in the "Common Bacteria System Identification Manual", the screening of the preservation number: CGMCC No.8341 was identified, and the colony morphology observation was carried out on PB medium. After the bacterial strain was streaked and inoculated, the Incubate at 28°C for 36 hours to observe the colony morphology. The colonies of the strain with the preservation number CGMCC No.8341 are nearly round, milky white in color, relatively dry and wrinkled on the surface, dull and opaque, eroded on the edges, sunken in the middle, easy to pick, the front and back of the colony or the edge and the central part consistent color (e.g. image 3 shown).

[0050] After picking and culturing the colony for 24 hours, after Gram staining, observe under the 100 times oil lens of the optical microscope, as shown in Figure 4 As shown, it can be seen und...

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Abstract

The invention relates to an antagonistic bacterium for controlling leaf spot of peanut and a microbial preparation thereof, belonging to the field of biological control on crop disease. The invention provides a Bacillus amyloliquefaciens BA-KA1 of which the collection number is CGMCC No.8341 and a microbial inoculant containing the same, and also provides application of the Bacillus amyloliquefaciens or the microbial inoculant in control on leaf spot of peanut. According to the invention, the Bacillus amyloliquefaciens BA-KA1 of which the collection number is CGMCC No.8341 and the microbial inoculant thereof have obvious antagonistic action on leaf spot bacteria of peanut, and can control occurrence of leaf spot of peanut; the control efficiency is above 70%. The use of the strain and the microbial preparation thereof can reduce application of chemical pesticides in peanut production processes, thus having favorable ecological and social benefits.

Description

technical field [0001] The invention relates to a bacillus amyloliquefaciens BA-KA1 strain which has antagonistic effect on peanut leaf spot and a microbial agent capable of preventing and treating peanut leaf spot, belonging to the field of biotechnology. Background technique [0002] Peanut leaf spot is the general term for leaf black spot and brown spot. The disease mainly occurs on the leaves, petioles, stipules, and stems are also affected. Generally, the disease starts on the lower leaves and gradually spreads to the upper leaves. The leaves spread, and when the disease is severe, disease spots can be formed on the stems, petioles, fruit needles, etc. There are clear yellow halos around the leaf spots on the front of the leaves, and the leaves are yellowish brown to dark brown. [0003] At present, farmers do not know enough about peanut leaf spot disease, and think that the leaf spot disease in the later stage of peanut growth basically does not affect the yield, so i...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
Inventor 黄亚丽马宏刘昆昂贾振华宋聪赵芊黄媛媛宋水山李书生
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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