A detachable chromatographic column

Abstract

The invention provides a detachable chromatographic column, belonging to the field of biological purification instruments. According to the chromatographic column provided by the invention, the column body is provided with scales, so that the operating personnel can conveniently control the filler height and feed amount; and the top end of the column body is provided with a U-shaped screen, so that a buffer solution, a sample solution, an eluate and other liquids can conveniently flow down along the wall of the column body, thereby avoiding splashing. The column head and column tail are detachable from the column body; and the chromatographic column can be assembled before chromatography, and can be detached after chromatography, so that the column head, column tail and column body can be respectively cleaned, sterilized and the like. The chromatographic column has the advantages of simple structure and favorable protein purification effect, and is convenient to operate.

Description

technical field [0001] The invention belongs to biological purification equipment, in particular to a detachable chromatographic column. Background technique [0002] At present, the separation and purification of various compound compositions, natural products, and traditional Chinese medicines are mainly achieved by column chromatography, using a chromatography column, and through a column chromatography process. In traditional column chromatography, the mixed compound is placed on the top of the glass column bed, so that the eluent (mobile phase) composed of the mixed solvent passes through a vertical glass column equipped with an adsorptive medium material (stationary phase) under the action of gravity, Separate and obtain each relatively pure product (pure product), its principle is: because each component in the mixed compound has different affinities to the stationary phase and mobile phase in the chromatographic column, so select the appropriate stationary phase and ...

AI Technical Summary

Problems solved by technology

The current common glass chromatography columns have the following deficiencies: on the one hand, in the filling of glass chromatography columns and the filling of samples of separated substances, different fillers and sample ratios are often used according to different fillers and different substances, so as to achieve the optimum Good separation effect while saving packing, and packing. The ratio of the sample is usually referred to its volume ratio, but currently the commonly used glass column does not have a scale, which often requires the operator to use experience to measure visually or use other measuring tools, which is very troublesome; on the other hand, in the chromatography process, it is often necessary to grasp the The separation medium is the migration speed in the filler, and then the speed of the effluent is adjusted according to the migration speed, so as to save chromatography time while achieving the best separation effect. However, the current chromatography column has no scale, and it is often at different time points Marking the position of a certain chromatographic ribbon is not accurate enough. At the same time, the marks marked by the marking pen may be washed away due to the leakage when adding eluent, and need to be marked again, which is very inconvenient

[0004] In addition, when the current chromatographic column is injected, the sample solution flows directly into the column from the middle of the top of the chromatographic column, which easily causes the filler in the column to splash onto the inner wall of the chromatographic column

Since the packing material used for chromatography is often relatively expensive, the packing material splashed on the inner wall is not fully utilized in the chromatography, which affects the chromatographic effect

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