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SSR core primer group developed based on sesame complete genomic sequence and application

A whole genome and core primer technology, applied in the fields of molecular biology and genetic breeding, can solve the problems of consuming a lot of manpower and material resources, and achieve the effect of easy statistics, high polymorphism, and stable and reliable markers

Active Publication Date: 2014-03-26
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are generally a large number of SSR sites in the genome. If all the SSR sites in the genome are analyzed one by one for the tested germplasm, it will take a lot of manpower and material resources.

Method used

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  • SSR core primer group developed based on sesame complete genomic sequence and application
  • SSR core primer group developed based on sesame complete genomic sequence and application
  • SSR core primer group developed based on sesame complete genomic sequence and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Development of sesame SSR core primer set

[0030] 1. Materials and methods:

[0031] 1.1 Materials

[0032] The test materials are 31 representative sesame resources: 31 sesame resource materials from 13 countries are rich in types, including single stem and branch; single flower and three flower; tall stem and short stem; yellow stem and green stem; Purple flower, light purple flower; green petiole, purple petiole; seed coat white, brown, black and other sesame germplasm and varieties, basically covering the main ecological types and main agronomic traits of sesame, reflecting as many sesame germplasm as possible diversity, with high genetic diversity (see Table 1).

[0033] Form 131 Sesame resource material information

[0034]

[0035]

[0036] 1.2 Research Methods

[0037] (1) Genomic DNA extraction by CTAB method

[0038] The total DNA of leaves was extracted by the CTAB method, and the specific steps were as follows:

[0039] a. An appropriate amo...

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PUM

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Abstract

The invention discloses a SSR core primer group developed based on sesame complete genomic sequence and application. The primer group comprises 32 pairs of primers, and the nucleotide sequences are shown as the sequence SEQ ID NO. 1-64. The obtained SSR core primer group has the advantages of being capable of furthest reflecting the heredity diversity of sesame germplasm resource materials, high in polymorphism, good in repeatability, stable in mark, easy for discrimination statistics and the like, is applicable to the fields of sesame variety identification, sesame variety heredity pedigree analysis and sesame variety germplasm resource heredity diversity evaluation and the like; and the primer group can be utilized to perform sesame heredity diversity analysis, and the heredity genetic relationship of a to-be-tested material can be furthest accurately reflected.

Description

technical field [0001] The invention belongs to the technical field of molecular biology and genetic breeding, and specifically relates to a set of SSR core primers developed based on the whole genome sequence of sesame and its application. Background technique [0002] Sesame is one of the important edible oil crops in my country, with high application value and nutritional value. Traditional sesame sesame oil, cold-rolled sesame cooking oil and various sesame foods are indispensable and irreplaceable special foods in people's life. Sesame is rich in functional components such as sesamin, sesamol, and sesamolin, which have various functions such as antiviral, bactericidal, and anticancer, and are widely used in medicine and clinical practice. [0003] Molecular markers based on DNA polymorphisms are gradually becoming a powerful tool for analyzing biological genetic diversity, kinship and identifying important genes. The most widely used molecular markers in crop genetic a...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 张秀荣魏鑫王林海张艳欣高媛丁霞
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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