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Compound bacterium and application thereof

A technology for compounding bacteria and halomonas, applied in the field of compounding bacteria, can solve the problems of farmers' economic loss, harm to breeding animals, ineffectiveness, etc., and achieve the effect of overcoming slow growth or death of bacteria and quickly purifying water quality.

Active Publication Date: 2015-06-10
北京龙科方舟生物工程技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the middle and late stages of aquaculture, due to excessive feeding of feed or water pollution, the content of ammonia nitrogen and nitrite in the aquaculture water is too high, causing serious harm to the aquaculture animals.
However, some water quality regulating products currently available in the market are slow or ineffective, causing unnecessary economic losses to farmers.

Method used

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  • Compound bacterium and application thereof
  • Compound bacterium and application thereof
  • Compound bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, screening and identification of bacteria

[0042] 1. Pseudomonas stutzeri 150-2

[0043] (1) Pseudomonas stutzeri 150-2 was screened out from 436 strains of aerobic denitrifying bacteria collected from the aquaculture area in Changping, Beijing, the aquaculture water bodies of surrounding counties and cities, and the soil samples of crops

[0044] This strain can be cultivated in LB medium, the medium components are as follows: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000mL, prepared into 1L liquid medium, pH 7.0, cultured at 37°C with constant temperature and shaking.

[0045] The screening process of the bacteria is as follows: 436 strains of bacteria obtained from water samples and soil samples were isolated and cultured in BTB selective medium for 24 hours, specific single colonies were picked, inserted into ammonia nitrogen medium for domestication, and then used ammonia nitrogen medium Expand the cultivation, measure the...

Embodiment 2

[0098] Embodiment 2, the composite application research of three bacterial strains

[0099] The three strains of bacteria 150-4, 150-2 and PSD screened in Example 1 were compounded to determine the effect of the compounded bacteria on water purification. The specific steps are as follows:

[0100] 1. There are 5 experimental groups and 1 control group breeding boxes in the fish pond (the volume of each breeding box is 50L), and each breeding box has 10 healthy Oni tilapia weighing about 50g.

[0101] 2. Use sodium nitrite reagent to adjust the nitrite content to about 0.4 mg / L in the water body of each group of breeding boxes.

[0102] 3. Add the bacterial strains or compound bacterial strains of the experimental group and the solution of the control group to each breeding box respectively, so that the number of viable bacteria of each kind of bacteria in the breeding box of each experimental group reaches 10 5 cfu / ml.

[0103] 4. Take the water body of each breeding box to de...

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PUM

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Abstract

The invention discloses a compound bacterium and application thereof, and particularly discloses bacillus amyloliquefaciens with the preservation number of CGMCC (China general microbiological culture collection center) No. 7364. According to the compound bacterium and the application thereof, three bacteria which are used for purifying water quality and have low requirements for nutritional conditions are screened out for compounding use, the defect that water quality purifying bacteria which are put into the water body grow slowly or die due to disproportion of the nutritional conditions after a water quality regulating product is put into the water body is overcome, and the water quality is purified quickly.

Description

technical field [0001] The invention relates to a compound bacterium and its application. Background technique [0002] In the middle and late stages of aquaculture, due to excessive feeding of feed or water pollution, the content of ammonia nitrogen and nitrite in the aquaculture water is too high, causing serious harm to the aquaculture animals. However, some commercially available products for regulating water quality are slow to take effect or have no effect, causing unnecessary economic losses to farmers. Contents of the invention [0003] The object of the present invention is to provide a compound bacterium and its application. [0004] A Bacillus amyloliquefaciens provided by the present invention has a preservation number of CGMCC No.7364. [0005] A compound bacterium also belongs to the scope of protection of the present invention, consisting of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) with the preservation number CGMCC No.7364 and Halomonas cam...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07C12R1/38C12R1/01
Inventor 薛新升谯仕彦丁修良王炜宏郑玉琦
Owner 北京龙科方舟生物工程技术有限公司
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