A cultivation device and method for detecting the activity of soil hydrocarbon oxidizing bacteria

A culturing device and a technology for hydrocarbon oxidizing bacteria, which are applied in measurement devices, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problems affecting the accuracy of hydrocarbon oxidizing microorganisms, etc., and solve the problems of soil permeability and soil moisture. Contradictions, Guaranteed Reliability, and Improved Efficiency

Active Publication Date: 2015-10-21
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This directly affects the accuracy of the determination of the activity of hydrocarbon oxidizing microorganisms

Method used

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  • A cultivation device and method for detecting the activity of soil hydrocarbon oxidizing bacteria
  • A cultivation device and method for detecting the activity of soil hydrocarbon oxidizing bacteria
  • A cultivation device and method for detecting the activity of soil hydrocarbon oxidizing bacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Such as figure 1 As shown, the cultivation device for detecting the activity of soil hydrocarbon oxidizing bacteria includes a detection bottle 1 and an injection needle that can be inserted into the detection bottle 1 . The test bottle here is a serum bottle with a rubber stopper and a perforated outer cap. All materials used can be sterilized by high temperature.

[0047] Implementation steps:

[0048] (1) For the test bottle, take a 50ml serum bottle and its corresponding rubber stopper and outer cover with holes. The bracket is processed with high-temperature cooking-resistant plastic film. The machine is folded and welded in half every 4 cm along the length direction, and each welding section is separated by 1-2 cm. After welding, it is curled along the length direction, clamped with nickel, and put into a serum bottle. After it is loosened inside, adjust the its flat. The loading net can be made of degreased gauze by cutting a piece of gauze with a side length...

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Abstract

The invention discloses a culture device and method for activity detection of soil hydrocarbon oxidation bacteria. The culture device comprises a detection bottle (1) and an injection needle capable of being inserted into the detection bottle (1), wherein the detection bottle (1) is a container or a device capable of forming an airtight space; a bracket (5) is arranged inside the detection bottle; water is formed below the bracket; a loading net (4) is paved and covered on the bracket; sealing of the detection bottle comprises sealing of a rubber plug (3) and a perforated cover (2). When the device is used, a gas is injected into or sucked from the detection bottle by the injection needle penetrating through the rubber plug. The culture device is improved on the basis of activity detection of the conventional soil hydrocarbon oxidation bacteria, the humidity inside the culture device can be increased, the gas permeability of a soil sample can be ensured, the methane utilization rate of methane-oxidizing bacteria and the hydrocarbon consumption rate of hydrocarbon oxidation bacteria are improved, and the reliability of activity detection is ensured. Thus, reliable technical means is supplied for application of a microbiological exploration technology.

Description

technical field [0001] The present invention relates to a device for detecting the activity of soil hydrocarbon-oxidizing bacteria in the field of soil microbial activity detection, and furthermore, relates to a cultivation device and method for detecting the activity of soil hydrocarbon-oxidizing bacteria used in detecting the activity of hydrocarbon-oxidizing bacteria . Background technique [0002] In the past two decades, with the improvement of microbial detection technology and the increase of international and domestic energy demand, microbial oil and gas exploration technology has shown strong vitality. The activity of gaseous hydrocarbon oxidizing bacteria in soil is an exploration index as important as its quantity. How to improve the accuracy of gaseous hydrocarbon oxidizing bacteria activity detection is one of the keys to improving microbial oil and gas exploration technology. [0003] The current general method for the determination of the activity of soil hyd...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/24C12Q1/04G01N30/02
CPCC12M23/08C12M23/38C12M23/48C12M29/00C12M41/36C12Q1/04
Inventor 任春黄欣汤玉平许科伟高国平杨帆赵克斌
Owner CHINA PETROLEUM & CHEM CORP
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