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Method for extracting portulace oleracea polysaccharide by use of high-voltage pulsed electric field

A technology of high-voltage pulsed electric field and purslane, which is applied in the field of natural medicinal chemistry, can solve problems such as no relevant reports, and achieve huge social and economic benefits, simple process, and high extraction rate

Active Publication Date: 2014-03-26
郯城县经开区医药发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no relevant report on the research on the extraction of polysaccharides in purslane by high-voltage pulsed electric field

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Purslane polysaccharide extraction process.

[0017] (1) Purslane pretreatment.

[0018] Take 3 kg of freshly picked purslane to remove impurities, rinse with water and drain the water, vacuum-dry at a temperature of 50°C to constant weight, crush, pass through a 60-mesh sieve, and place in a desiccator for later use.

[0019] (2) Purslane polysaccharides were extracted by high-voltage pulsed electric field.

[0020] Take 150g of pretreated purslane powder, add 10 times the weight of water, adjust the pH value to 7.0, and place it in a bipolar square wave high-voltage pulsed electric field for extraction at a temperature of 40°C. The pulsed electric field strength is 10kV / cm, and the frequency 200Hz, the processing time is 0.5 h, and the extract is collected.

[0021] (3) Alcohol precipitation, washing and drying of the polysaccharide extract.

[0022] Centrifuge the extract obtained in step (2), concentrate the supernatant under reduced pressure to a certain volu...

Embodiment 2

[0026] 1. Purslane polysaccharide extraction process.

[0027] (1) Purslane pretreatment.

[0028] Take 3 kg of freshly picked purslane to remove impurities, rinse with water and drain the water, vacuum-dry at a temperature of 50°C to constant weight, crush, pass through a 60-mesh sieve, and place in a desiccator for later use.

[0029] (2) Purslane polysaccharides were extracted by high-voltage pulsed electric field.

[0030] Take 150g of pretreated purslane powder, add 12 times the weight of water, adjust the pH value to 8.0, and extract it in a bipolar square wave high-voltage pulsed electric field at a temperature of 40°C, characterized in that the pulsed electric field intensity is 20kV / cm , the frequency is 400Hz, the processing time is 3.0 h, and the extract is collected.

[0031] (3) Alcohol precipitation, washing and drying of the polysaccharide extract.

[0032] Centrifuge the extract obtained in step (2), concentrate the supernatant under reduced pressure to a ce...

Embodiment 3

[0036] 1. Purslane polysaccharide extraction process.

[0037] (1) Purslane pretreatment.

[0038] Take 3 kg of freshly picked purslane to remove impurities, rinse with water and drain the water, vacuum-dry at a temperature of 50°C to constant weight, crush, pass through a 60-mesh sieve, and place in a desiccator for later use.

[0039] (2) Purslane polysaccharides were extracted by high-voltage pulsed electric field.

[0040] Take 150g of pretreated purslane powder, add 15 times the weight of water, adjust the pH value to 8.0, and extract it in a bipolar square wave high-voltage pulsed electric field at a temperature of 40°C, characterized in that the pulsed electric field intensity is 40kV / cm , the frequency is 800Hz, the processing time is 4.0 h, and the extract is collected.

[0041] (3) Alcohol precipitation, washing and drying of the polysaccharide extract.

[0042] Centrifuge the extract obtained in step (2), concentrate the supernatant under reduced pressure to a ce...

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PUM

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Abstract

The invention discloses a method for extracting portulace oleracea polysaccharide by use of a high-voltage pulsed electric field, which comprises the following steps: (1) performing pretreatment on fresh portulace oleracea; (2) putting the portulace oleracea powder obtained in the step (1) in a bipolar square-wave high-voltage pulsed electric field for extraction, and performing solid-liquid separation to obtain extracting solution; (3) adding a proper amount of 95% ethanol into the extracting solution, re-dissolving the precipitate in dissolved water and adding 95% ethanol for alcohol precipitation for 3 times, washing the precipitate with absolute ethyl alcohol, anhydrous ethyl ether and acetone, and performing vacuum drying to obtain the portulace oleracea polysaccharide extract. In the method for extracting portulace oleracea polysaccharide by use of high-voltage pulsed electric field technology, disclosed by the invention, due to low-temperature extraction, the structure of portulace oleracea polysaccharide is maintained to the greatest degree, and the product quality is improved; meanwhile, the process is advanced, the extraction time is short, the extraction rate is high, integration of low-temperature extraction and sterilization is realized, and thermosensitive substances are not degraded for sure. The method is a good method for extracting portulace oleracea polysaccharide, and the obtained polysaccharide can be applied to the fields of food, cosmetics and the like.

Description

technical field [0001] The invention belongs to the technical field of natural medicinal chemistry. Background technique [0002] Purslane is a weed belonging to the family Portulaca oleracea. It is a wild plant with the same source of medicine and food. Its resources are very rich and are distributed in most parts of my country. Purslane is sour in taste and cold in nature. It has the effects of clearing away heat, detoxifying and reducing swelling. A plant with great development potential. Purslane is rich in a variety of nutrients and medicinal ingredients, such as polysaccharides, flavonoids, alkaloids, anthraquinone, etc. Modern pharmacological research shows that purslane has anti-oxidation, antibacterial, anti-tumor, blood fat-lowering, and immune-enhancing functions. effect. [0003] Purslane polysaccharide is one of the main functional components of purslane. Purslane polysaccharide has antibacterial effect, can lower blood sugar and blood fat, resist aging and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 杨安树熊江花陈红兵吴志华李欣佟平
Owner 郯城县经开区医药发展有限公司
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