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Nucleic acid amplification apparatus and nucleic acid analysis apparatus

A nucleic acid amplification device and nucleic acid analysis technology, applied in the field of nucleic acid analysis devices, to achieve the effect of temperature uniformity

Active Publication Date: 2014-03-12
HITACHI HIGH-TECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, there are cases where a plurality of temperature control modules that can individually control the temperature are mounted on one device

Method used

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  • Nucleic acid amplification apparatus and nucleic acid analysis apparatus
  • Nucleic acid amplification apparatus and nucleic acid analysis apparatus
  • Nucleic acid amplification apparatus and nucleic acid analysis apparatus

Examples

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specific example 1

[0070] image 3 A specific example of a nucleic acid amplification device that corrects the absolute values ​​of temperatures of a plurality of temperature control modules 1 using one temperature calibration sample is shown. image 3 The device configuration shown is for the figure 2 The configuration of the device shown is a configuration shown in more detail.

[0071] In the case of this embodiment example, four temperature control modules 1 capable of individually controlling the temperature are arranged along the outer edge of the disc-shaped rotating disk 22 . The rotary disk 22 here corresponds to the base of the embodiment example 1. As shown in FIG. The rotary disk 22 is made of a material excellent in heat insulation. Therefore, the mutual interference of temperature among multiple temperature control modules can be ignored.

[0072] The rotary disk 22 is fixed to an unillustrated rotary shaft, and can freely rotate both clockwise and counterclockwise as indicate...

specific example 2

[0082] Figure 4 Another device configuration example of a nucleic acid amplification device that corrects the temperature of the temperature control module 1 using one type of temperature calibration sample is shown. Figure 4 The device configuration shown is also for figure 2 The shown device configuration is a device configuration shown in more detail.

[0083] exist image 3 In the case of the device configuration shown, the number of real-time fluorescence measurement units 3 is reduced relative to the number of temperature control modules 1 . Therefore, either one of the base on which the temperature control module 1 is placed and the real-time fluorescence measurement unit 3 is fixed, and the rotation of the other is controlled.

[0084] However, in the case where the temperature control module 1 and the real-time fluorescence measurement part 3 correspond one-to-one and there are multiple temperature control modules 1 and real-time fluorescence measurement parts 3...

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Abstract

In conventional techniques, when a temperature measurement probe that has been corrected is used for the correction of absolute values of temperatures of temperature control blocks of which the temperatures can be controlled individually, there are temperature differences of up to 0.5 DEG C among the temperature control blocks. In the present invention, in contrast, a melting temperature of a temperature correction sample that is housed in each of reaction vessels corresponding to the temperature control blocks is measured as a measurement melting temperature. Subsequently, comparison is made between the measurement melting temperature corresponding to each of the temperature control blocks and a reference melting temperature for the temperature correction sample, and the absolute value of the temperature of each of the temperature control blocks is corrected on the basis of the value of the resultant difference.

Description

technical field [0001] The present invention relates to a nucleic acid amplification device equipped with a plurality of temperature control modules capable of individually controlling temperatures, and a nucleic acid analysis device using the nucleic acid amplification device as part of the device. Background technique [0002] The temperature control module needs to control the absolute value of its temperature correctly at the target temperature. Conventionally, in the measurement of the absolute value of the temperature of the temperature control module, the following method is used: when the measured temperature is different from the target temperature using a calibrated temperature measurement probe, correction is made so that the measured temperature matches the target temperature. . In general, the limit accuracy of temperature correction using a calibrated temperature measurement probe is ±0.25°C. [0003] However, there are cases where a plurality of temperature ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00
CPCB01L2200/148C12M1/00B01L7/52B01L2300/024B01L2300/0829B01L2300/1822
Inventor 前田耕史杉山千枝庄司义之石泽雅人佐野稔
Owner HITACHI HIGH-TECH CORP
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