Making method for artificial hybrid liriodendron seeds
A technology of hybridizing Liriodendron chinensis and artificial seeds, applied in the fields of biotechnology and agricultural engineering, to achieve the effects of simple source of materials, reduction of domestication and transplanting process, and convenient transportation and storage
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[0025] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.
[0026] This implementation just makes hybrid Liriodendron artificial seed according to the following process.
[0027] 1. Culture of hybrid Liriodendron cotyledon embryo
[0028] When the somatic embryos of Liriodendron chinensis grow to cotyledon embryos, the cotyledon embryos are picked out from the culture medium and inoculated aseptically on the cotyledon embryo growth medium for culture. The composition of cotyledon embryo growth medium is: MS+IAA0.5mg / L+ZT2mg / L+glutamic acid 400mg / L+3.0% sucrose+0.7% agar. MS is Murashige and Skooge medium, IAA is indole acetic acid, and ZT is zeatin. Culture conditions: light for 14 hours, light intensity 35μmol·m -2 ·s -1 . The light source is LED red light. The pH of the medium was 5.8.
[0029] 2. Production of hybrid Liriodendron cotyledon embryo capsules
[0030] The hybrid Liriodendr...
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