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Making method for artificial hybrid liriodendron seeds

A technology of hybridizing Liriodendron chinensis and artificial seeds, which is applied in the field of biotechnology and agricultural engineering, and achieves the effects of convenient transportation and storage, small size and saving seeds

Inactive Publication Date: 2015-04-22
YUNNAN JIANGSHI TECH CO LTD +1
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  • Claims
  • Application Information

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Problems solved by technology

However, the implementation of this technology still cannot meet the current demand for hybrid tulip tree seedlings in production, so it is necessary to develop a more efficient production technology for hybrid tulip tree seedlings

Method used

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  • Making method for artificial hybrid liriodendron seeds
  • Making method for artificial hybrid liriodendron seeds

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Embodiment Construction

[0025] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0026] This implementation just makes hybrid Liriodendron artificial seed according to the following process.

[0027] 1. Culture of hybrid Liriodendron cotyledon embryo

[0028] When the somatic embryos of Liriodendron chinensis grow to cotyledon embryos, the cotyledon embryos are picked out from the culture medium and inoculated aseptically on the cotyledon embryo growth medium for culture. The composition of cotyledon embryo growth medium is: MS+IAA0.5mg / L+ZT2mg / L+glutamic acid 400mg / L+3.0% sucrose+0.7% agar. MS is Murashige and Skooge medium, IAA is indole acetic acid, and ZT is zeatin. Culture conditions: light for 14 hours, light intensity 35μmol·m -2 ·s -1 . The light source is LED red light. The pH of the medium was 5.8.

[0029] 2. Production of hybrid Liriodendron cotyledon embryo capsules

[0030] The hybrid Liriodendr...

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Abstract

The invention discloses a making method for artificial hybrid liriodendron seeds. The making method comprises the steps of cultivating hybrid liriodendron cotyledon embryos, making hybrid liriodendron cotyledon embryo capsules, preparing artificial hybrid liriodendron seed coats and the like. The artificial seeds made by the making method can replace natural tree seeds, so that the liriodendron foresting period is shortened; furthermore, the making method cannot be limited by seasons and can be applied to large-scale and large-batch high-efficiency production of the artificial hybrid liriodendron seeds; sufficient seedlings are supplied to large-scale planting of hybrid liriodendrons.

Description

technical field [0001] The invention belongs to the fields of biotechnology and agricultural engineering, and in particular relates to a method for making artificial seeds of hybrid tulip tree. Background technique [0002] Liriodendron, also known as mandarin wood, duck foot wood, nine-layer bark, maple lotus, etc., is a plant of the genus Liriodendron in the family Magnoliaceae. There are only two species of this genus, namely, Liriodendron tulipifolia distributed in the middle and northern subtropical regions of my country, extending from Zhejiang and Fujian in the east to Yunnan in the southwest, and the Sino-Vietnamese border, and Liriodendron tulipifera in the eastern United States. Liriodendron genus occupies a relatively primitive position in the taxonomic status of angiosperms. Although the distribution area is wide, it is mostly distributed in small groups at present. Liriodendron, which is distributed in China, has been listed as a national second-class rare and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 吴沿友赵玉国乔韡轶姜猛王金田刘彩赵宽邢德科
Owner YUNNAN JIANGSHI TECH CO LTD
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