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Method of preparing calcium gluconate by composite immobilized enzyme

A technology of calcium gluconate and immobilized enzymes, applied in the direction of immobilization on or in inorganic carriers, on/in organic carriers, chemical industry, etc., can solve the problem of low enzyme immobilization rate and recovery rate, low price, etc. High cost and other issues, to achieve the effect of low price, increased reuse times, and improved reuse rate

Active Publication Date: 2014-02-26
SHANDONG XIWANG SUGAR
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the price and cost of the immobilization material are high, and the immobilization rate and recovery rate of the enzyme are low

Method used

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  • Method of preparing calcium gluconate by composite immobilized enzyme
  • Method of preparing calcium gluconate by composite immobilized enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Prepare 1500mL sodium alginate and silica gel mixed solution, the concentration of sodium alginate is 1.0%-1.5%, the concentration of silica gel is 1.0%-2.0% (calculated as sodium silicate), sterilize at 121°C and cool it down. Glucose oxidase equivalent to 2.5‰ of the glucose quality in the reaction solution and 7.5‰ of catalase were added to it, and fully stirred evenly; using a syringe, mix sodium alginate and silica gel mixed with glucose oxidase and catalase Drop the mixed solution solution into the 6% calcium chloride solution that has been sterilized and cooled to fix the shape, and let it stand still for 4 hours; use anhydrous glucose to prepare 3.5L glucose solution with a concentration of 10% (W / V), put it in the In the 5L reaction tank, first add 32.4g of calcium carbonate solid; add the formed immobilized enzyme into the reaction tank, and set the reaction conditions as follows: sterile air is introduced, the stirring speed is 200-300 r / min, and the enzym...

Embodiment 2

[0035](1) Prepare 16L mixed solution of sodium alginate and silica gel, the concentration of sodium alginate is 1.0%-1.5%, the concentration of silica gel is 1.0%-2.0% (calculated as sodium silicate), sterilize at 121°C and cool down. Glucose oxidase and 1% catalase, which is equivalent to 2.5‰ of the glucose mass in the reaction solution, were added to it, and fully stirred evenly; using a peristaltic pump with multiple syringe needles, mixed glucose oxidase and catalase Drop the mixed solution of sodium alginate and silica gel into the sterilized and cooled 5% calcium chloride solution to fix the shape, and let it stand still for 6 hours; use anhydrous glucose to prepare 70L glucose solution with a concentration of 10% (W / V), and disinfect After cooling, put it in a 100L reaction tank, first add 640g of calcium carbonate solid; add the formed immobilized enzyme into the reaction tank, set the reaction conditions as follows: feed sterile air, stirring speed 200-300 r / min, enzy...

Embodiment 3

[0038] A comparative example is as follows:

[0039] Using anhydrous glucose, prepare 70L of glucose solution with a concentration of 10% (W / V), put it in a 100L reaction tank after disinfection and cooling, add glucose oxidase and peroxidase, and add 640g of calcium carbonate solid first; set the reaction conditions as : Sterile air was introduced, the stirring speed was 200-300 r / min, and the enzyme reaction temperature was 33°C; after 6 hours of reaction, add the remaining 1300g of calcium carbonate solid (slightly excessive calcium carbonate), and continue the reaction for 20 hours, and the reaction liquid After the reducing sugar concentration drops below 0.2%, stop the reaction, stop ventilation, stir, take out the reaction solution, add activated carbon at 0.7% (W:V) to decolorize at 75 ° C for 1 hour, filter to obtain the filtrate, and the vacuum degree is 0.095MPa Under the conditions, evaporate at 60°C until the dry matter percentage is 70%, add 1% calcium gluconate ...

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Abstract

The invention provides a method of preparing calcium gluconate by a composite immobilized enzyme. Improvement is made on the basis of present methods which produce the calcium gluconate by a free composite enzyme method. Glucose oxidase and catalase are added into a mixed solution of sodium alginate and silica gel, and then are added dropwise into a calcium carbonate solution to be immobilized and moulded. The glucose oxidase and the catalase are immobilized to achieve enzyme liquid separation, thus improving the repeating utilization ratio of the glucose oxidase and the catalase and reducing the using cost of the enzyme. A calcium gluconate product produced by the method provided by the invention is high in purity, and conforms to requirements of the country about energy conservation and environment protection of enterprises at present.

Description

technical field [0001] The invention belongs to the technical field of calcium gluconate production, in particular to a method for preparing calcium gluconate with immobilized compound enzymes. Background technique [0002] Calcium gluconate is a polyhydroxy organic acid salt with a wide range of uses. Due to its non-toxic and wide source of raw materials, it is mainly used as a calcium fortifier and nutrient, buffer, curing agent, and chelating agent for food. Its application prospects Very broad. As an important food additive, food-grade calcium gluconate has a bright application prospect in food. It can be used as a nutritional supplement, quality improver, etc.; pharmaceutical-grade calcium gluconate can be used as a calcium supplement for the human body. Calcium is the content in the body. The largest inorganic substance, necessary to maintain the normal function of human nerve, muscle, skeletal system, cell membrane and capillary permeability. Calcium ions are import...

Claims

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Application Information

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IPC IPC(8): C12P7/58C12N11/14C12N11/10C12N11/04
CPCY02P20/50
Inventor 王棣李伟王一王居亮孙学谦李秋红夏颖颖邓智王茜李树谢登玉
Owner SHANDONG XIWANG SUGAR
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