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Application of Tibet rhodiola crenulata extract in preparing medicament for resisting intestinal inflammation

A technology of Rhodiola grandiflora and its extracts, which can be applied in the direction of anti-inflammatory agents, drug combinations, antipyretics, etc., and can solve the problems that anti-intestinal inflammation research has not been reported.

Inactive Publication Date: 2014-02-26
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no report on the anti-intestinal inflammation of Rhodiola rosea extract

Method used

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  • Application of Tibet rhodiola crenulata extract in preparing medicament for resisting intestinal inflammation
  • Application of Tibet rhodiola crenulata extract in preparing medicament for resisting intestinal inflammation
  • Application of Tibet rhodiola crenulata extract in preparing medicament for resisting intestinal inflammation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The preparation of embodiment 1 Rhodiola rosea extract

[0014] Weigh 20g of the dried root of Rhodiola daflora, crush it, soak it in 200mL deionized water for 12h, heat and boil on low heat for 3h, filter, then add 200mL deionized water and extract twice with the same method, combine the three extracts and concentrate to 200mL, that is to obtain 0.1g / ml (10%) Rhodiola rosea extract, and store it sealed at 4°C for later use. The content of salidroside in the extract of Rhodiola daflora was not less than 0.5% as determined by high performance liquid chromatography, which met the quality standard of "Chinese Pharmacopoeia" 2010 edition (Part 1).

Embodiment 2

[0015] Example 2 Effect of Rhodiola rosea extract on the survival rate of postfly after treatment with fungi Bassiana, M.luteus and inflammatory factor SDS

[0016] Preparation of B. bassiana spore liquid: spread B. bassiana on fresh LB solid medium, and culture it statically at 25°C for 7 days. The spores on the medium were collected with sterile PBS and filtered to measure the spore concentration. After the spores were centrifuged, the supernatant was discarded, and 5% sucrose solution (control group) or Rhodiola grandiflora extract containing 5% sucrose (experimental group) was added to the precipitate to prepare A 600 =40 spore solution, stored at 4°C for later use.

[0017] Preparation of M.luteus bacterial liquid: M.luteus (LB medium, 37°C) was inoculated into 200mL liquid medium for overnight expansion culture, the bacterial liquid was centrifuged at 4000r / min, 25°C for 10min, the supernatant was discarded, and 5% Sucrose solution (control group) or Rhodiola grandis ex...

Embodiment 3

[0020] Example 3 Effect of Rhodiola rosea extract on intestinal epithelial cells of fly after SDS treatment

[0021] In order to explore the deeper mechanism of Rhodiola grandiflora extract improving the survival rate of Drosophila after SDS treatment, this experiment used 7-Aminoactinomycin (7-Aminoactionnomycin D, 7-AAD) staining to analyze the death of intestinal epithelial cells Condition. As a nucleic acid dye, 7-AAD can only enter dead cells and combine with its DNA, and can produce red fluorescence after excitation.

[0022] Randomly collect 30-40 female fruit flies that have been eclosion for 3-4 days, and feed them with SDS (0.6%) solution containing 5% sucrose for 96 hours. The complete intestinal tract was isolated under a microscope, stained with 7-AAD for 30 minutes, fixed with paraformaldehyde for 10 minutes, stained with DAPI for 10 minutes, mounted with glycerol, observed and photographed under a fluorescent microscope. About 10 Drosophila intestines were iso...

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Abstract

The invention provides an application of Tibet rhodiola crenulata extract in preparing a medicament for resisting intestinal inflammation. The rhodiola crenulata extract is obtained by adopting a water extraction method, and is subjected to a drosophila intestinal inflammatory injury repairing experiment. Results prove that the rhodiola crenulata extract has excellent anti-inflammation and anti-oxidation activities, and can be used for preparing medicaments for resisting intestinal inflammation.

Description

technical field [0001] The invention relates to the application of the extract of Rhodiola rosea daflora in the preparation of anti-intestinal inflammation medicine, and relates to the field of traditional Chinese medicine and the technical field of natural immunity of fruit flies. Background technique [0002] The intestinal mucosa is exposed to a large amount of different antigenic substances such as food, bacteria, and viruses every day. In the intestinal tract, the host needs to develop immune responses to pathogenic microorganisms and immune tolerance to food antigens and other normal intestinal lumen contents. The mucosal barrier functions. Intestinal mucosal immunity against pathogenic microorganisms can be divided into innate immunity and acquired immunity, in which innate immunity forms the basis of the intestinal mucosal barrier. Intestinal innate immunity is an important defense mechanism of the intestine and the host's first line of defense against pathogenic mi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/41A61P1/00A61P29/00
Inventor 金丽华朱彩霞关法春兰小中
Owner NORTHEAST FORESTRY UNIVERSITY
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