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Leiocassis longirostris skin collagen antioxidation peptide, and preparation method and application thereof

A technology of antioxidant peptides and collagen, which is applied in the field of food biochemistry to achieve the effects of low price, high efficiency and wide sources

Active Publication Date: 2014-02-12
黑龙江仙芙生物医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there is no report on the research on the antioxidant peptide of catfish skin collagen

Method used

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  • Leiocassis longirostris skin collagen antioxidation peptide, and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0032] Treat catfish skin with 0.1mol / L NaoH for 2 hours at a liquid-to-material ratio of 40mL / g, wash with distilled water until neutral, add n-hexane to degrease, after degreasing, use 0.3mol / L lactic acid solution to extract collagen at 40°C , the extraction time is 7 hours, the ratio of liquid to solid is 40mL / g, dialyze in distilled water for 24 hours after the extraction, freeze-dry, prepare the lyophilized collagen into a 2% (w / v, g / mL) solution, and adjust the pH to 7.5 , use protease alcalase (Novozymes food grade alcalase 2.4L) to enzymolyze at 40°C for 60min, the amount of enzyme added is 2000U / g. After drying, the catfish skin collagen antioxidant peptide was obtained. The antioxidant activity of the antioxidant peptide product is: IC of scavenging DPPH free radicals 50 It is 0.86mg / mL, the IC of the ability to scavenge hydroxyl radicals 50 0.91mg / mL, chelated Fe 2+ Capable IC 50 1.35mg / mL.

Embodiment 2

[0034]Treat catfish skin with 0.1mol / L NaoH for 2.5h according to the ratio of liquid to material at 50mL / g, wash with distilled water until neutral, add n-hexane to degrease, after degreasing, extract collagen with 0.4mol / L lactic acid solution, extraction temperature is 45 ℃, extraction time 9h, liquid-to-solid ratio 50mL / g, dialyze in distilled water for 24h after extraction, freeze-dry, prepare freeze-dried collagen into a 2.5% (w / v, g / mL) solution, adjust pH to 8.0, use protease alcalase (Novozymes food grade alcalase 2.4L) to enzymolyze at 45°C for 90min, the amount of enzyme added is 3000U / g. After freeze-drying, the catfish skin collagen antioxidant peptide was obtained. The antioxidant activity of the antioxidant peptide product is: IC of scavenging DPPH free radicals 50 It is 0.53mg / mL, the IC of the ability to scavenge hydroxyl radicals 50 0.72mg / mL, chelated Fe 2+ Capable IC 50 1.01mg / mL.

Embodiment 3

[0036] Treat catfish skin with 0.1mol / L NaoH for 3 hours at a liquid-to-material ratio of 60mL / g, wash with distilled water until neutral, add n-hexane to degrease, after degreasing, extract collagen with 0.5mol / L lactic acid solution, and the extraction temperature is 50°C , the extraction time is 11 hours, the ratio of liquid to material is 60mL / g, dialyze in distilled water for 24 hours after the extraction, freeze-dry, prepare the lyophilized collagen into a 3% (w / v, g / mL) solution, and adjust the pH to 9.5 , use protease alcalase (Novozymes food grade alcalase 2.4L) to enzymolyze at 50°C for 120min, the amount of enzyme added is 4000U / g, after the enzymolysis is completed, the enzyme is inactivated in a boiling water bath, the supernatant is collected by centrifugation, concentrated in vacuum, and frozen After drying, the catfish skin collagen antioxidant peptide was obtained. The antioxidant activity of the antioxidant peptide product is: IC of scavenging DPPH free radic...

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Abstract

The invention relates to a Leiocassis longirostris skin collagen antioxidation peptide, and a preparation method and application thereof. The preparation method comprises the following steps: removing alkali-soluble proteins from Leiocassis longirostris skin with an alkali solution, degreasing with n-hexane, extracting collagen with a lactic acid solution, dialyzing, carrying out freeze-drying to obtain the collagen, hydrolyzing the obtained collagen with proteinase under proper enzymolysis conditions, and carrying out freeze-drying on the enzymolysis product to obtain the Leiocassis longirostris skin collagen antioxidation peptide. The Leiocassis longirostris skin collagen antioxidation peptide has favorable activity, the IC50 for removing DPPH free radicals is 0.53-0.86 mg / ml, the IC50 for removing hydroxy free radicals is 0.72-0.91 mg / ml, and the IC50 for chelating Fe<2+> is 1.01-1.35 mg / ml.

Description

technical field [0001] The invention relates to the field of food biochemistry and can be used as a functional additive in food, cosmetics and medicines, in particular to a catfish skin collagen antioxidant peptide, a preparation method thereof and an application thereof. Background technique [0002] Many chronic human diseases such as cancer, coronary heart disease and Alzheimer's disease are related to free radicals. Free radicals in food are usually removed by synthetic antioxidants, which are harmful to human health. The antioxidant peptides in it are green, safe and efficient, and are suitable for use as antioxidants for human health care. [0003] Collagen from animal tissues has been reported to have extremely high antioxidant activity, which is mainly related to the fact that collagen contains a large number of highly active antioxidant peptides. At present, there are Chinese patents on collagen antioxidant peptide activity and its preparation Reports on methods, s...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/14A61K38/01A61P39/06
Inventor 吴琼英贾俊强颜辉桂仲争
Owner 黑龙江仙芙生物医疗科技有限公司
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