Method for cultivating dominant-green-shin white feather broiler strain based on molecular assistant selection
A white-feathered broiler, molecular-assisted technology, applied in the field of animal husbandry, etc., can solve the problems of long cycle, size impact of group expansion, cumbersome test cross, etc., achieve high production performance, speed up the breeding process, and avoid cumbersome effects
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[0035] The breeding method of the blue-shin dominant white-feathered broiler strain based on molecular assisted selection in the present embodiment comprises the following steps:
[0036] (1) Use Gushi pure line roosters as the male parent and Avian hen as the female parent to carry out crossbreeding to produce F1 generation chickens, eliminate a small number of individuals with colored feathers that appear in the hybridization, and select the F1 generation of blue feathers from the F1 white feather population. White-shined male and female hens;
[0037] (2) The F1 generation is self-crossed to obtain the F2 generation, and the F2 generation of green shin and white feather male and female chickens are selected;
[0038] (3) Using the RFLP-PCR method of artificially introducing PvuII to determine the genotype of the F2 generation of blue-shined white-feathered male and female hens, specifically including the following steps:
[0039] ① Collection of samples
[0040] Take bloo...
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