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Method for preparing crocetin from cape jasmine

A technology of saffron acid and gardenia, which is applied in the field of preparation of saffron acid, can solve the problems of high price and shortage of saffron medicinal materials, and achieve the effects of increasing the yield, improving the utilization rate of raw materials, and shortening the process

Active Publication Date: 2014-01-29
SHAANXI YUANBANG BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention provides a preparation method of crocin acid derived from gardenia to solve the problem of shortage and high price of saffron medicinal materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: A preparation method of gardenia-derived crocetin, comprising the following steps in sequence.

[0022] 1. Extraction: 100g of gardenia fruit, extracted twice with 8 times the amount of 70% ethanol, combined, concentrated until no alcohol smell, spray-dried to obtain 18.6g of powder.

[0023] 2. Enzyme hydrolysis: add 3 times water to the above powder, 1% mixed enzyme (SPE-005, SPE-007A, Aspergillus niger, ratio 1:1:3). Enzyme hydrolyzed at 45°C and pH5 for 24 hours. Among them, SPE-005 mainly contains cellulase, pectinase, xylanase and B-glucanase; SPE-007A mainly contains B-glucosidase, rhamnosidase and xylanase Enzyme, B-glucanase, cellulase, hemicellulase.

[0024] 3. Alkaline extraction: After the enzymatic hydrolysis is completed, add 5 times the amount of 1% sodium hydroxide solution to the reactant, stir and extract at 70°C for 1 hour, filter, and neutralize the lye after filtration to pH3-4 with dilute hydrochloric acid, there will be coarse Th...

Embodiment 2

[0026] Embodiment 2: A preparation method of gardenia-derived crocetin, comprising the following steps in sequence.

[0027] 1. Extraction: 200g of gardenia fruit, extracted twice with 8 times the amount of 70% ethanol, combined, concentrated until no alcohol smell, sprayed with powder to obtain 37.8g of powder.

[0028] 2. Enzyme hydrolysis: add 5 times of water to the above powder, and 1.5% mixed enzyme (SPE-005, SPE-007A, Aspergillus niger, ratio 1:1:3). Enzymatic hydrolysis at 40°C, pH5.2 for 24 hours.

[0029] 3. Alkaline extraction: After the enzymatic hydrolysis is completed, add 5 times the amount of 1% sodium hydroxide solution to the reactant, stir and extract at 70°C for 1 hour, filter, and neutralize the lye after filtration to pH3-4 with dilute hydrochloric acid, there will be coarse The product was precipitated, centrifugally filtered, centrifugally precipitated and dried to obtain 1.55 g of crocetin with a content of 53.3%.

[0030] 4. Crystallization: Weigh 1...

Embodiment 3

[0031] Embodiment 3: A preparation method of gardenia-derived crocetin, comprising the following steps in sequence.

[0032] 1. Extraction: 200g of gardenia fruit, extracted twice with 10 times the amount of 70% ethanol, combined, concentrated until no alcohol smell, sprayed with powder to obtain 38.4g of powder.

[0033] 2. Enzyme hydrolysis: add 5 times of water to the above powder, and 1.5% mixed enzyme (SPE-005, SPE-007A, Aspergillus niger, ratio 1:1:3). Enzymatic hydrolysis at 45°C, pH5.5 for 24 hours

[0034] 3. Alkaline extraction: After the enzymatic hydrolysis is completed, add 5 times the amount of 1% sodium hydroxide solution to the reactant, stir and extract at 70°C for 1 hour, filter, and neutralize the lye after filtration to pH3-4 with dilute hydrochloric acid, there will be coarse The product was separated out, centrifugally filtered, precipitated and dried to obtain 1.69g of 52.3% crocetin.

[0035] 4. Crystallization: Weigh 1.65g of crocetin in the above 3,...

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Abstract

The invention provides a method for preparing crocetin from cape jasmine. The method comprises the following steps: extracting a cape jasmine raw material; performing enzymolysis on the raw material to obtain powder, and adding mixed enzyme; performing alkali extraction and enzymolysis, adding a reactant sodium hydroxide, stirring and extracting for 1 hour, filtering, separating a coarse product, and refiltering to obtain crocetin with content of over 50 percent; crystallizing to obtain crocetin, adding absolute ethyl alcohol, refluxing for 2 hours, cooling and crystallizing, filtering and baking to obtain the crocetin product with content of over 95 percent. The method can be used for solving the problems of drug shortage and high price of saffron.

Description

technical field [0001] The invention relates to a preparation method of crocetin derived from gardenia. Background technique [0002] Crocetin acid is one of the active ingredients of saffron stigma, which has an unsaturated conjugated polyenoic acid structure and belongs to carotenoids. Crocetin has various physiological functions such as lowering cholesterol, inhibiting tumor growth, and inhibiting liver poisoning. In addition, crocetin is a skin singlet oxygen scavenger, a collagen synthesis stimulator and a skin immunity booster, all of which require high-purity crocetin. However, the yield of saffron stigma is extremely low, and its resources are extremely limited, so it is difficult to cultivate it in a large area in China, which makes it expensive and known as "plant gold". [0003] The dried and ripe fruit of the traditional Chinese medicine Gardenia is rich in yellow pigment, and its main components are Crocin and Crocetin. Therefore, it can be considered to prepa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C57/13C07C51/42
CPCC07C51/42C07C57/13
Inventor 张永利张文雪赵江李艳寇玉锋
Owner SHAANXI YUANBANG BIO TECH
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