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Preparation method of composite strain in microbe fertilizer

A technology of microbial fertilizer and compound strains, which is applied in the field of preparation of compound strains in microbial fertilizers, can solve the problems of apple discoloration, drug residues, apple browning, etc., so as to prevent apple oxidation, maintain strain activity, and increase synergy. Effect

Active Publication Date: 2014-01-08
SHANDONG AIFUDI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, apples are one of the most popular and popular fruits in people's daily life. Apples are prone to discoloration, softening and rot, especially apple browning is the most common problem. Low temperature, air conditioning and chemical Preservatives are currently the most effective way to keep apples fresh, but there are common problems such as high cost and drug residues

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  • Preparation method of composite strain in microbe fertilizer

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Effect test

Embodiment １

[0024] A preparation method of composite strains in microbial fertilizer, 10 parts of polyacrylamide, 5 parts of matrine, 3 parts of ascorbic acid, 3 parts of EDTA, ZnCl 2 5 parts and 100 parts of microbial agent, and the rest are water.

[0025] The preparation steps are:

[0026] (1) Inoculate yeast, Bacillus licheniformis, Bacillus subtilis, photosynthetic bacteria, Streptomyces flavinus, and Bacillus lateralosporus into the primary culture medium respectively, and activate and cultivate them into primary strains; inoculate the primary strains respectively In the secondary medium, prepare the secondary single strain.

[0027] The primary culture medium of Streptomyces luteus is 1000 parts by weight of potato juice, 30 parts by weight of sucrose, 20 parts by weight of agar, the pH value is 7-8, and the temperature is 25-30 ° C; the secondary medium is 1000 parts by weight of potato juice, poly 1 weight part of acrylamide, 0.5 weight part of matrine, 0.5 weight part of asco...

Embodiment 2

[0036] A preparation method of composite strains in microbial fertilizer, 20 parts of polyacrylamide, 3 parts of matrine, 3 parts of ascorbic acid, 4 parts of EDTA, ZnCl 23 parts and 150 parts of microbial inoculants, and the rest are water.

[0037] (1) Inoculate yeast, Bacillus licheniformis, Bacillus subtilis, photosynthetic bacteria, Streptomyces flavinus, and Bacillus lateralosporus into the primary culture medium respectively, and activate and cultivate them into primary strains; inoculate the primary strains respectively In the secondary medium, prepare the secondary single strain.

[0038] The primary culture medium of Streptomyces luteus is 1000 parts by weight of potato juice, 50 parts by weight of sucrose, 15 parts by weight of agar, the pH value is 7-8, and the temperature is 25-30 ° C; the secondary medium is 1000 parts by weight of potato juice, poly 2 parts by weight of acrylamide, 0.3 parts by weight of matrine, 0.3 parts by weight of ascorbic acid, 0.4 parts ...

Embodiment 3

[0047] A preparation method of composite strains in microbial fertilizer, 15 parts of polyacrylamide, 4 parts of matrine, 3 parts of ascorbic acid, 3 parts of EDTA, ZnCl 2 4 parts and 50 parts of microbial inoculants, and the rest are water.

[0048] (1) Inoculate yeast, Bacillus licheniformis, Bacillus subtilis, photosynthetic bacteria, Streptomyces flavinus, and Bacillus lateralosporus into the primary culture medium respectively, and activate and cultivate them into primary strains; inoculate the primary strains respectively In the secondary medium, prepare the secondary single strain.

[0049] The primary medium of Streptomyces luteus is 1000 parts by weight of potato juice, 20 parts by weight of sucrose, 25 parts by weight of agar, the pH value is 7-8, and the temperature is 25-30 ° C; the secondary medium is 1000 parts by weight of potato juice, poly 1.5 parts by weight of acrylamide, 0.4 parts by weight of matrine, 0.3 parts by weight of ascorbic acid, 0.3 parts by wei...

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Abstract

The invention discloses a preparation method of a composite strain in microbe fertilizer. The preparation method comprises the following steps of respectively carrying out individual fermentation on strains in a composite microbial agent to obtain single-bacterium liquids of the strains, mixing the single-bacterium liquids and carrying out fermentation on the mixed bacterium liquid to obtain the agent of the composite strain. The composite strain fertilizer obtained by the preparation method can effectively keep strain activity and has strong strain cooperativity and high fertilizer efficiency.

Description

technical field [0001] The invention relates to the technical field of microbial fertilizers, in particular to a method for preparing composite strains in microbial fertilizers. Background technique [0002] There are a variety of microbial organic fertilizers on the market now, and today, as people are increasingly pursuing a higher quality of life, the development of efficient and safe fertilizers has become a development trend. Most of the existing fruit tree fertilizers can cause drug residues, many of which have strong toxic and side effects on the human body, and the cost is high and the production process is complicated. Due to the excessive use of chemical fertilizers and the continuous planting of crops, the soil began to compact in a large area, the beneficial bacteria in the soil became less and less, and soil-borne diseases such as heavy cropping and root-knot nematodes became more and more serious; Streaks, poor disease resistance, frequent occurrence of variou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C12N1/14C12N1/20C12R1/10C12R1/125C12R1/465
Inventor 车春玲
Owner SHANDONG AIFUDI BIOLOGICAL TECH
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