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Cultivation method and application of cordyceps militaris

A cultivation method and technology for Cordyceps militaris, applied in the field of Cordyceps militaris cultivation, can solve the problems that the anti-tumor effect is not very obvious and cannot reach people, etc.

Active Publication Date: 2014-01-01
杭州佗鹊堂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, most of the Cordyceps militaris currently on the market only pursues the content of cordycepin, resulting in insignificant anti-tumor effects and failing to meet people's expectations

Method used

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  • Cultivation method and application of cordyceps militaris
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  • Cultivation method and application of cordyceps militaris

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Embodiment Construction

[0020] The present invention will be further described in detail below in conjunction with the examples, but not limited thereto.

[0021] In the method, the silkworm chrysalis after silk reeling is used as the main raw material, and the rice is used as an auxiliary material to cultivate the Cordyceps militaris, which can be harvested for two seasons, and each bottle can obtain 1 g of dry weight Cordyceps militaris each time.

[0022] The tumor-bearing mice mentioned in the examples are C57 inoculated with Lewis lung cancer. Mice C57 mice are ordinary-grade experimental animals, both male and female, purchased from the Experimental Animal Center of the Academy of Military Medical Sciences, and the experimental animal quality certificate number is SCXK-(Army) 2007-004. The lewis lung cancer, the source of the tumor, was preserved in our laboratory. Cyclophosphamide tablets, aluminum-plastic packaging, each containing 50 mg cyclophosphamide, 50 mg total saponins from ginseng st...

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Abstract

The invention provides a cultivation method and application of cordyceps militaris. The method includes the steps that a culture medium is prepared by mixing dry silkworm chrysalis meal with an intensity of 20g / L, rice meal with the intensity of an intensity of 180g / L, monopotassium phosphate with an intensity of 1.2g / L, sodium dihydrogen phosphate with an intensity of 1g / L and the balance of water, and sealing, sterilization and cooling are carried out; second, a cordyceps militaris strain is inoculated, wherein the density of the strain is 3-5pellets / mL, and the volume ratio of the culture medium to the strain is 50:1; cultivation is carried out, wherein the cultivation includes dark cultivation and light cultivation; sporocarp is harvested. The cordyceps militaris cultivated through the method is made into medicine, C57 mice inoculated with the lewiss lung carcinomas are used as experimental subjects and dosed with the medicine according to the standard that the administration dosage of the cordyceps militaris in the medicine is 5g / kg, the C57 mice are dosed with the medicine one time per day, and the suppression ratio of the lewiss lung carcinomas is 19.6% according to detection after the C57 mice are continuously dosed with the medicine for 10 days.

Description

technical field [0001] The invention relates to the technical field of Cordyceps militaris cultivation, in particular to a method for cultivating Cordyceps militaris and the application of the Cordyceps militaris cultivated by the method in the preparation of medicines for treating lung cancer. Background technique [0002] Cordyceps militaris (Cordyceps militaris), also known as Cordyceps militaris, belongs to the subphylum Ascomycota, Ergotaceae, and genus Cordyceps of the fungal kingdom. It is a medicinal fungus. Cordyceps militaris contains sugar, fat and protein, in addition, it also contains a variety of amino acids, trace elements and vitamins. A variety of physiologically active components in Cordyceps militaris have pharmacological effects such as anti-tumor, antibacterial, anti-oxidation, liver protection, and immunity enhancement. And its tumor-related active substances include cordycepin, polysaccharides, cordycepic acid, sterols, selenium, carotenoids, protein...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G1/00
Inventor 陈剑清舒特俊
Owner 杭州佗鹊堂生物科技有限公司
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