Method for preparing pulled type cervical smear

Abstract

The invention relates to a method for preparing a pulled type cervical smear. The method comprises the following steps: (1) oscillating a specimen through an oscillator; (2) centrifugating a part of the oscillated specimen until the specimen is layered into supernatant liquid and a cell layer; (3) removing the supernatant liquid, sucking the cell layer, dripping the cell layer on a glass slide, laminating the glass slide with another glass slide, and then pulling the glass slides to the opposite directions; (4) fixing and dyeing so as to prepare the smear. Compared with a conventional Pap smear method, the method provided by the invention has the advantages of high specimen satisfaction, high smear quality and the like. Compared with a liquid base cytology method, the method provided by the invention has the advantages of simplicity in operation, uniform tiling of the prepared smears, non-overlapping of cells, clear background, no precious equipment, low cost and the like, and is convenient to popularize. Compared with a human papillomavirus-deoxyribose nucleic acid (HPV-DNA) detection method, the method provided by the invention has the advantages of no use of precious equipment, low inspection cost, simplicity in operation, convenience in popularization and the like.

Description

technical field [0001] The invention relates to a method for preparing a cervical smear, and more specifically, to a method for preparing a pull-tab type cervical smear. Background technique [0002] At present, some preparation methods of Pap smears have low sample satisfaction and poor smear quality, which is not conducive to observation; some require expensive equipment and complicated operation steps, which are not conducive to popularization. [0003] Existing existing realization scheme most similar to the present invention has: traditional Pap smear method, liquid-based cytology examination method, HPV-DNA detection method, but all there is deficiency: [0004] 1. The satisfaction of Pap smear is low and the quality of the smear is poor. The main reasons are: 1. The diseased cells on the sampler are not all transferred to the glass slide; 2. The cells in the smear are unevenly distributed and accumulated too much , Mucus, blood, or inflammatory cells cover abnormal c...

AI Technical Summary

Problems solved by technology

[0004] 1. The satisfaction of Pap smear is low and the quality of the smear is poor. The main reasons are: 1. The diseased cells on the sampler are not all transferred to the glass slide; 2. The cells in the smear are unevenly distributed and accumulated too much , where mucus, blood, or inflammatory cells cover abnormal cells

[0005] 2. The liquid-based cytology method has the following disadvantages: 1. Specimens with excessive mucus and blood must be treated specially; 2. Sedimentation method, cells are often distributed in multiple layers, and abnormal cells often overlap, which affects observation; 3. It takes a lot of financial, manpower and material resources to carry out routine maintenance and maintenance of special equipment

[0006] 3. The equipment and supporting consumables required by HPV-DNA detection method are expensive and the operation is cumbersome