Multiple microsatellite identification system for carrying out litopenaeus vannamei family identification, and application thereof

Abstract

The present invention relates to a multiple microsatellite identification system for rapidly and efficiently carrying out litopenaeus vannamei family identification, and belongs to the field of molecule assisted breeding in genetic breeding. Specifically 15 microsatellite sites with high polymorphism are screened to carry out primer design, primers are divided into two primer systems according to primer annealing temperatures and amplification product lengths, the primers in every group are subjected to different fluorescence labeling according to the fragment length, system optimization is performed to obtain the optimal amplification system so as to carry out microsatellite typing on the candidate parents and the filial generation through the multiple microsatellite identification system, and family identification is performed by using the typing data of the 15 sites, such that efficiency is rapid, the result is accurate and reliable, and good identification effects are further provided for families with close kinship.

Description

technical field [0001] The invention relates to a molecular assisted breeding technique for genetic breeding, in particular to a multiple microsatellite identification system for fast and efficient identification of Litopenaeus vannamei family lines. Background technique [0002] Litopenaeus vannamei is the largest shrimp farmed in my country and even in the world. Its output accounts for 60% of the world's prawn production and 80% of my country's prawn production. In the breeding work of Penaeus nalime, in the breeding process, knowing accurate pedigree information is of great significance for reducing inbreeding and speeding up the breeding process. Therefore, the work of family identification has attracted the attention of scientific researchers. Wang Hongxia et al. 6 pairs of primers were selected from the high-performance microsatellite primers to identify the polyculture families. It was found that the probability of finding the real mother from 20 candidate female shrim...

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Problems solved by technology

This high-efficiency, accurate and low-cost microsatellite typing system is used in many terrestrial and aquatic organisms (Miao Guidong, Du Min, Yang Jingfeng, Xu Ying, Fan Tingjun, Chen Songlin: Paternity identification of turbot Establishment and application of microsatellite multiplex PCR technology. Journal of Ocean University of China (Natural Science Edition) 2011(Z1): 97-106. Application. Acta Aquatica Sinica 2013 (02): 207-215), but there is no report in the family identification of Litopenaeus vannamei. At the same time, because multiple microsatellite sites can be analyzed, the accumulative identification accuracy of multiple sites can effectively improve the identification accuracy

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