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Quality testing method of Baihe Ganoderma lucidum

A quality detection method, detection method technology, applied in the direction of measuring devices, material separation, analysis of materials, etc., to achieve high efficiency, good separation effect, and good recovery rate

Inactive Publication Date: 2015-09-30
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Although the above-mentioned documents disclose some detection methods of Ganoderma lucidum, there is no report on the method of comprehensively detecting the active ingredients of Ganoderma lucidum, and then achieving quality control.

Method used

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  • Quality testing method of Baihe Ganoderma lucidum
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  • Quality testing method of Baihe Ganoderma lucidum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Establishment of HPLC fingerprint of Ganoderma lucidum medicinal material.

[0044] 1 Instruments and reagents

[0045] 1.1 Instrument

[0046] Agilent1200 High Performance Liquid Chromatograph (Agilent Technologies Co., Ltd., USA): including online vacuum degasser (G-1322A), high-pressure quaternary gradient pump (A-1311A), standard autosampler (G-1312A), intelligent Column oven (G-1316A), full-wavelength detector (G-1313A), Agilent1200 chromatographic workstation (Agilent Technologies, Inc., USA), and diode array detector.

[0047] 1.2 Reagents

[0048] Methanol, acetonitrile (U.S. Fisher Scientific Reagent Company, chromatographically pure); Methanol (Guangdong Guanghua Technology Co., Ltd., analytically pure, batch number: 21120116); 95% ethanol (Xilong Chemical Co., Ltd., analytically pure, batch number: 120427); Ethyl acetate (Xilong Chemical Co., Ltd., analytically pure, batch number: 120401); chloroform (Chengdu Kelong Chemical Reagent Factory, anal...

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Abstract

The invention discloses a quality detecting method of a rhinacanthus nasutus medicinal material. The quality detecting method is characterized by comprising a fingerprint detecting method and a content measuring method of the rhinacanthus nasutus medicinal material, wherein the fingerprint detecting method comprises the step: measuring by using an HPLC (High Performance Liquid Chromatography), wherein 12 characteristic peaks are available; the content measuring method comprises the steps: selecting components with relatively high contents and representativeness, and measuring the components by using the HPLC, wherein the components are extracted and separated from rhinacanthus nasutus, and the umbelliferone separated and extracted from the rhinacanthus nasutus is used as an quantitative index. The method is simple and convenient to operate; the various measured components are good in separation degree, relatively good in repeatability and relatively high in precision degree, stability and recovery rate.

Description

technical field [0001] The invention relates to the field of quality control of traditional Chinese medicines, in particular to a quality detection method of Ganoderma lucidum medicinal material. Background technique [0002] The establishment of a quality control system for traditional Chinese medicine must be based on the characteristics of traditional Chinese medicine. Chinese medicine has its own unique theoretical system and has made great contributions to the reproduction of the Chinese nation. Traditional Chinese medicine is a medicinal substance under the guidance of Chinese medicine theory, and it is also a practical source of material for creating new medicines with Chinese characteristics. The quality control and guarantee of traditional Chinese medicine is the core issue in the modernization, industrialization and internationalization of traditional Chinese medicine. In recent years, with the development of my country's traditional Chinese medicine science and t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 谢丽莎刘寿养龚志强黄茂春
Owner GUANGXI UNIV OF CHINESE MEDICINE
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