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An adjustable droplet generating device

A generating device and adjustable technology, which is applied in the field of adjustable droplet generating device, can solve the problems of droplet preparation and detection equipment transfer, etc., and achieve the effect of simple production and meeting application requirements

Active Publication Date: 2016-06-08
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problems solved by the present invention: firstly, the size of the prepared droplet can be adjusted in multiple dimensions to obtain droplets of different sizes, so as to meet different application requirements; secondly, to overcome the need to transfer between the existing droplet preparation and detection equipment To solve the problem, a droplet generating device based on detection equipment is provided, and the generated droplets can directly enter the detection equipment, realizing the integration of microfluidic technology and detection equipment

Method used

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  • An adjustable droplet generating device

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Embodiment 1

[0023] The specific embodiment of the present invention is the preferred embodiment of basic embodiment, specifically as follows:

[0024] In this embodiment, the capillary is a capillary glass tube with an outer diameter of 365 μm, the tip is drawn to a diameter of 10 μm, the tee joint is a standard joint for liquid chromatography, and the variable diameter tube can be drawn by itself. The dispersed phase is PCR reaction reagent, and the continuous phase is silicone oil. The liquid flow is driven by positive air pressure, and water-in-oil droplets can be formed at the tip of 3. The pressure driven by the liquid is increased from 0.1Mpa to 0.4Mpa, and the size of the droplet is reduced from 60um to about 15um. When the liquid driving pressure is reduced below 0.1Mpa, the droplet size will further increase, and may even exceed 100um. If the pressure is too low to push the liquid, it won't produce droplets.

Embodiment 2

[0026] In this embodiment, the capillary is a capillary glass tube with an outer diameter of 365 μm, the tip is drawn to a diameter of 10 μm, the tee joint is a standard joint for liquid chromatography, and the variable diameter tube can be drawn by itself. The dispersed phase is PCR reaction reagent, and the continuous phase is silicone oil. The liquid flow is driven by positive air pressure, and water-in-oil droplets can be formed at the tip of 3. Keep the gas driving pressure constant at 0.3Mpa, change the relative position of 3 and 2, or fix 3 and change the relative position of 2 and 1 to obtain droplets of different sizes. The droplet size is generally between 30um and 80um. A position adjustment knob can also be added at the connection between 3 and 1, so that the relative position of 3 and 2 can be controlled more conveniently. It is also possible to drive with positive pressure at terminals 3 and 4, and at the same time apply negative pressure at the outlet of 5 to d...

Embodiment 3

[0028] In this embodiment, the relative positions of 1, 2, and 3 are fixed, the driving pressure is maintained at 0.2Mpa, and the capillary tip of 3 is replaced with a 2um tip. At this time, the droplet size can be reduced to below 10um. If the tip of 3 is replaced with one of about 50um, the diameter of the obtained droplet is usually above 100um.

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Abstract

A capillary-based tunable droplet generation device. The generating device can be driven by positive pressure or negative pressure to generate nanoliter to picoliter-scale droplets; compared with the traditional microfluidic chip droplet generating device, the generating device does not require microfluidic preparation process and is easy to manufacture. The size of the generated droplets can be adjusted in multiple dimensions. And the droplet generation technology can be seamlessly embedded into the existing biological detection process.

Description

technical field [0001] The invention belongs to the technical field of microfluid applications, in particular to an adjustable droplet generating device. Background technique [0002] Microfluidics is the basis of a new generation of digital biological detection technology, and the reason why it is called digital is that there are nanoliter or even picoliter-level droplets suspended in microfluidics. These droplets are a set of microreactors. It not only greatly reduces the amount of reagents used, but also speeds up the detection speed, which is the core and key of the new generation of detection technology. At present, droplets are generally generated by syringe pumps in microfluidic chips based on planar photolithography technology PDMS. The generated droplet suspension needs to be transferred to a droplet detection device such as flow cytometry. That is, current microfluidic droplet generation techniques and biomolecular detection workflows are independent of each othe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N30/06
Inventor 罗昭锋何立群吴平
Owner UNIV OF SCI & TECH OF CHINA
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