Transgenic hamster and preparation method thereof

Abstract

The invention relates to a preparation method of transgenic hamster. According to the following technical scheme, the method comprises the following steps of: enabling an oocyte donation rat and male rat to be normally mated, taking out the fertilized ovum, culturing the fertilized ovum in a special culture solution for the hamster, microinjecting a green fluorescent protein gene, and then transfusing the fertilized ovum to surrogate rat for breeding. The preparation method can be used for overcoming the defect that the transgenic hamster cannot be cultured by virtue of a technology in the prior art.

Description

technical field [0001] The present invention relates to an invention in the field of genetic engineering, in particular to a transgenic hamster and a preparation method thereof. Background technique [0002] In recent decades, due to changes in human lifestyles, metabolic cardiovascular diseases (atherosclerotic cardiovascular and cerebrovascular diseases with glucose and lipid metabolism disorders) have surpassed tumors to become the disease with the highest mortality rate in humans. Therefore, extensive and in-depth research on metabolic cardiovascular diseases has come to an urgent period. Although such research can be carried out through direct investigation of clinical patients, since it is difficult to obtain samples of the cardiovascular system from patients, and it is impossible to apply various damaging experimental conditions to patients, the application of metabolic cardiovascular Animal models of the disease are essential to carry out corresponding research. ...

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Problems solved by technology

However, a follow-up report 3 years later mentioned that the company failed to successfully produce transgenic hamsters

Although they did not report the reason for the failure, they learned from exchanges with colleagues at home and abroad that there were research groups from the College of Animal Husbandry and Veterinary Medicine of Jilin University, the Yunnan Institute of Zoology, Hungary and France who tried to manipulate hamster embryos, but because the fertilized eggs of hamsters were in vitro There is a special "block of 2 cell development" phenomenon in the development process, so the fertilized eggs reinfused into the body cannot develop into mature hamster embryos

However, most of the fertilized eggs cultured in vitro stagnate at the two-cell stage, so the two-cell arrest is the main reason that hinders the successful production of transgenic hamsters.

[0008] Therefore, judging from the prior art, hamsters are of great value in the research of metabolic cardiovascular diseases, and the cost of raising and breeding hamsters is relatively low; however, the research on transgenic hamsters is still blank at home and abroad.

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