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CYP2D6 gene segment containing 4094C>A mutant, protein segment coded by same and application thereof

A technology of fragments and nucleic acid fragments, applied in the field of biology, can solve problems such as differences in drug efficacy, drug side effects, and insufficient treatment

Active Publication Date: 2013-12-11
蔡剑平
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] According to current clinical research, this polymorphism of CYP2D6 gene is the main reason for the significant difference in CYP2D6 enzyme activity among individuals, and individuals carrying different CYP2D6 genotypes can cause great differences in drug efficacy, and even serious Drug toxicity or inadequate treatment of

Method used

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  • CYP2D6 gene segment containing 4094C>A mutant, protein segment coded by same and application thereof
  • CYP2D6 gene segment containing 4094C>A mutant, protein segment coded by same and application thereof
  • CYP2D6 gene segment containing 4094C>A mutant, protein segment coded by same and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0069] Example 1: Identification of new mutation sites in human CYP2D6 gene

[0070] In this example, blood samples were collected from normal healthy people of the Han nationality, genomic DNA in the blood was extracted, and sequencing primers were designed to amplify and sequence the 9 exons of the CYP2D6 gene to analyze whether there were mutation sites in the CYP2D6 gene.

[0071] 1) DNA extraction:

[0072] Take a 5ml venous EDTA anticoagulated blood sample from the subject; then follow the common salting out method and / or use a special DNA extraction kit (purchased from Omega, USA) to extract the genomic DNA of the blood sample to be tested.

[0073] 2) PCR amplification:

[0074] Amplification primers were designed to amplify the 9 exon sequences of CYP2D6 gene in the obtained genomic DNA samples. The sequences of the amplification primer pairs are shown in Table 1.

[0075] Use 30μL PCR reaction system, including: 1×GC PCR buffer, 1.5mM MgCl 2 , 100ng of genomic DN...

Embodiment 2

[0089] Example 2: Analysis of enzyme metabolic activity in vitro

[0090] According to the existing research results, the metabolic activity of the wild type (*1 type) to various drugs is relatively high, while the metabolic activity of the *2 type is significantly lower than that of the wild type, and the metabolic activity of the *10 type is higher than that of the *10 type. Type 2 is lower (see literature 6, 7). Therefore, there is a consensus in the field that the metabolic activity of enzymes expressed by the same genotype on specific substrates can represent the metabolic activity on other substrate drugs. Thereby, according to the specific substrate metabolic activity data of the enzyme expressed by a certain genotype, the metabolic activity of the enzyme expressed by the genotype to other substrate drugs can be deduced (for example, the metabolic activity of the enzyme expressed by the genotype can be inferred). activity compared to the metabolic activity of the wild-...

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Abstract

The invention belongs to the field of biology and relates to a single base mutant at the 4094th site of CYP2D6 allele, wherein C at the site is mutated into A. The invention particularly relates to a nucleic acid segment containing the mutant site and a corresponding protein segment coded by the nucleic acid segment, a reagent and detection method for identifying the mutant site, application of the site, and especially application of identification of the site in medicine instruction.

Description

technical field [0001] The invention belongs to the field of biology and relates to a single base mutation at the 4094th position of CYP2D6 allele. More specifically, the present invention relates to a nucleic acid fragment containing the mutation site and its corresponding encoded protein fragment, a reagent for identifying the mutation site, a detection method and the application of identifying the site in guiding medication. Background technique [0002] Cytochrome P450 2D6 (CYP2D6) is one of the important members of the CYP enzyme family. Its gene is located on chromosome 22, including 9 exons, with a total length of 9432bp (GenBank registration number M33388, and the exon region is located at positions 1620-5909). The amount of cytochrome in the human body only accounts for 2% to 4% of the total amount of liver enzymes, but it participates in the metabolism of 20% to 30% of clinical drugs, such drugs include antidepressants, antiarrhythmic drugs, antipsychotic drugs, s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53C12N15/11C12N9/02C12Q1/68
Inventor 蔡剑平胡国新戴大鹏耿培武蔡杰
Owner 蔡剑平
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