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Application of Diaphorobacter bacterium in phenol removal of coking wastewater

A strain and phenol-reducing technology, applied in bacteria, water pollutants, chemical instruments and methods, etc., can solve the problems of different sources of phenol-reducing strains, different adaptability of wastewater, and inhibition of biochemical degradation of phenols.

Inactive Publication Date: 2013-12-04
CHINA UNIV OF MINING & TECH (BEIJING)
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, different phenol-degrading strains have different sources and have different adaptability to different wastewaters, so there are not many applications in the biological treatment of coking wastewater
On the other hand, coking wastewater is a complex mixed system, often containing a variety of organic pollutants and high concentrations of heavy metal ions, which will seriously inhibit the biochemical degradation of phenols by microorganisms

Method used

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  • Application of Diaphorobacter bacterium in phenol removal of coking wastewater
  • Application of Diaphorobacter bacterium in phenol removal of coking wastewater
  • Application of Diaphorobacter bacterium in phenol removal of coking wastewater

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Embodiment Construction

[0015] Collect activated sludge samples from the secondary sedimentation tank of the coking plant, put them into sterile sealed plastic pots, and bring them back to the laboratory for enrichment and screening.

[0016] 2. Medium formula required for cultivation and isolation identification

[0017] Beef extract peptone enrichment medium: peptone 10g, beef extract 3g, sodium chloride 5g, deionized water 1000ml, pH 7.0-7.2, phenol (according to requirement), solid medium is to add 15- 20g agar.

[0018] Specific formula of inorganic salt liquid medium: KH 2 PO 4 0.5g, NH 4 NO 3 1.0g, MgSO 4 0.2g, CaCl 2 0.2g, FeSO 4 ·7H 2 O 0.01g, MnSO 4 ·H 2 O 0.01g, phenol (according to the need to add), add distilled water to 1L, the solid medium is to add 15 ~ 20g agar to this medium.

[0019] The NaOH concentration used to adjust the pH value is 2 mol / L, and the HCl concentration is 0.5 mol / L.

[0020] 3. Screening, isolation and identification of phenol-degrading strains

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Abstract

The invention discloses phenol-degradation Diaphorobacter sp. bacterial strain P2 screened and separated from coking wastewater; 16S rDNA sequence similarity analysis shows that the homology of the P2 bacterial strain and a known Diaphorobacter 16S rDNA sequence is 99%; The Diaphorobacter P2 can degrade phenol high up to 1000 mg / L; influence of compound heavy metals on the phenol degrading efficiency of the P2 bacterial strain is: in order, Zn<2+> > Mn<2+> > Pb<2+>, and Pb<2+> has no significant influence on the phenol degrading efficiency of the Diaphorobacter P2 bacterial strain; and the P2 is inoculated in prepared wastewater in which the volume ratios of the coking wastewater to distilled water are respectively 1:2 and 1:1, and phenolic substances can be completely degraded in 4 days. The application of the Diaphorobacter bacterium in phenol removal of the coking wastewater provides materials and theoretical basis for the biological treatment of phenol pollution of coking wastewater and the development of bioremediating inoculants.

Description

Technical field: [0001] The invention relates to a phenol-degrading bacterial strain Diaphorobacter P2, which can tolerate heavy metals such as Mn, Zn and Pb, has the ability to degrade pyridine and o-cresol, and particularly relates to the biological treatment of phenolic organic pollutants in coking wastewater. Background technique: [0002] Phenol is an important organic chemical raw material, widely used in synthetic fiber, synthetic rubber, stone, medicine, pesticide, fragrance, dyestuff and paint. At the same time, it is also the main pollutant of waste water in gas washing, coking, synthetic ammonia, paper making, wood preservation and chemical industry, among which the waste water produced by coking is a typical refractory industrial waste water. Coking wastewater contains phenols, benzene series, amines, alkanes, quinoline and other organic substances, as well as sulfides, cyanides, oils and heavy metals (copper, manganese, zinc, nickel, lead, cadmium, etc.), phenol...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/34
Inventor 张玉秀柴团耀蒙小俊
Owner CHINA UNIV OF MINING & TECH (BEIJING)
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