Extraction method for pomegranate foline

A technology of pomegranate leaf element and extraction method, which is applied in the direction of chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of undiscovered industrial extraction method of pomegranate leaf element, and achieve short preparation period and large separation amount Effect

Inactive Publication Date: 2013-12-04
NANJING BIAOKE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] According to the literature search, there is no report on the industrial extraction method of pomegranate leaf element in China

Method used

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  • Extraction method for pomegranate foline

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Get 1000g of dry leaves of Macaranga tanarius, place in a flash extractor, add 7L of 75% ethanol solution as extraction solvent, extract voltage is 140V, extraction time is 90s, carry out flash extraction once, extract for 15min, filter to obtain extract, Recover the reagent under reduced pressure from the extract, put the concentrated solution on the HPD100 macroporous adsorption resin column, elute with water first, and then elute with 55% ethanol by volume ratio, collect 5 times the column volume of the ethanol eluate, pass the eluate through D201 type strongly basic styrene type anion exchange resin, elute with 0.5mol / L NaOH solution, collect the eluate, pass through D001 type strong acidic styrene type cation exchange resin column, collect the lower column liquid, concentrate and dry at low temperature Pomegranate leaf element white powder, content 84.6%, yield 70.6%, UV, IR, MS, 2 HNMR, 13 The data characterizing its physical properties such as CNMR are consisten...

Embodiment 2

[0025] Take 1000g of dried leaves of Macaranga tanarius, place them in a flash extractor, add 7L of 65% ethanol solution as the extraction solvent, extract the voltage at 160V, and extract for 100s, perform flash extraction twice, each time for 10min, and filter to obtain the extract , the extract is decompressed to recover the reagent, put the concentrated solution on the HPD100 type macroporous adsorption resin column, first elute with water, and then elute with 55% ethanol by volume ratio, collect 5 times the column volume ethanol eluate, and eluate the eluate Pass through the D201 type strongly basic styrene type anion exchange resin, elute with 0.4mol / L NaOH solution, collect the eluate, pass through the 001×4 type strong acidic styrene type cation exchange resin column, collect the lower column liquid, and store it at low temperature Concentrate and dry to get pomegranate leaf element white powder, content 85.3%, yield 64.7%, UV, IR, MS, 2 HNMR, 13 The data characterizi...

Embodiment 3

[0027] Take 1000g of dried leaves of Macaranga tanarius, place them in a flash extractor, add 7L of 55% ethanol solution as the extraction solvent, the extraction voltage is 150V, the extraction time is 60s, carry out flash extraction 3 times, each time for 5min, and filter to obtain the extract , the extract is decompressed to recover the reagent, put the concentrated solution on the HPD100 type macroporous adsorption resin column, first elute with water, and then elute with 55% ethanol by volume ratio, collect 5 times the column volume ethanol eluate, and eluate the eluate Pass through D202 type strongly basic styrene type anion exchange resin, elute with 0.5mol / L NaOH solution, collect the eluate, pass through D001 type strong acidic styrene type cation exchange resin column, collect the lower column liquid, concentrate and dry at low temperature That is to get pomegranate leaf element white powder, content 85.9%, yield 74.8%, UV, IR, MS, 2 HNMR, 13 The data characterizing...

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Abstract

The invention discloses an extraction method for pomegranate foline and belongs to the chemical field of natural medicines. The method comprises the following steps: taking dry leaves of macaranga tanarius as raw materials, putting into a flash type extractor; taking hydrous ethanol as an extraction solvent for flash type extraction; filtering to obtain an extract liquid, followed by concentration; putting concentrated solution on a big-hole adsorption resin column; eluting by using water and 50-65 percent ethanol in sequence; adopting an anion exchange resin column, performing eluting by the NaOH solution; collecting the eluant; collecting the off-column liquid by using the cation exchange resin column, and obtaining the pomegranate foline through decompression and concentration. The method adopts flash type extraction and resin separation technology, is efficient and environment-friendly, and therefore is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of extraction of natural substances, and relates to an extraction method of pomegranate leaf element. Background technique [0002] Pomegranate leaf element is a tannin compound isolated from the leaves of Macaranga tanarius (L.) Muell.et Arg., a plant of Euphorbiaceae (Euphorbiaceae), with a molecular formula of C 41 h 30 o 26 , the molecular weight is 938.67, and the structural formula is [0003] [0004] Modern research shows that pomegranate leaf element has anti-protein kinase C activity, IC50 is 4μM; inhibits topoisomerase II activity, IC50 is 0.2μM; inhibits hyaluronidase activity, and the inhibition rate is 96% at 10mM. [0005] According to literature search, there is no report on the industrial extraction method of pomegranate leaf element in China. Contents of the invention [0006] The purpose of the present invention is to provide an efficient and environment-friendly extraction method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H13/08C07H1/08
Inventor 张金芳万冬梅
Owner NANJING BIAOKE BIO TECH
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