A kind of black bone rattan compound extract and its preparation method and application
A kind of extract, the technology of the black bone rattan, applied in the field of the compound extract of the black bone rattan and its preparation and application, can solve the problems of inconvenient use for patients, low content of active ingredients, saving, etc.
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Embodiment 1
[0031] Embodiment 1 The preparation of the compound extract of Teng japonica of the present invention
[0032] prescription:
[0033] Black bone vine 50g, Gentiana 20g, Yuanhu 20g
[0034] Preparation:
[0035] (1) Weigh the raw material drug by weight, ultrasonically extract it with 50% v / v ethanol, combine the extracts, recover the ethanol until it has no alcohol smell, and obtain the crude extract;
[0036] (2) Take the crude extract, put it on a D101 macroporous resin column, first elute with water to remove impurities (20 times column volume), then elute with 20 times column volume 20% v / v ethanol, collect the ethanol eluate, reduce After being concentrated under pressure, freeze-dried to obtain the compound extract of Teng Teng.
[0037] For the determination method of total flavonoids and total saponins in the present invention, please refer to the ultraviolet spectrophotometry commonly used in this field; for the detection methods of gentiopicroside, glucoside and t...
Embodiment 2
[0038] Embodiment 2 The preparation of the compound extract of Teng japonica of the present invention
[0039] prescription:
[0040] Black bone vine 50g, Gentiana 20g, Yuanhu 20g
[0041] Preparation:
[0042] (1) Weigh the raw material drug by weight, ultrasonically extract it with 50% v / v ethanol, combine the extracts, recover the ethanol until it has no alcohol smell, and obtain the crude extract;
[0043] (2) Take the crude extract, put it on a D101 macroporous resin column, first elute with water to remove impurities (20 times column volume), and then use 20 times column volume 20%v / v, 40%v / v, 60% Eluted with v / v ethanol, collected 60% ethanol eluate, concentrated under reduced pressure, and freeze-dried to obtain the compound extract of Teng Teng.
Embodiment 3
[0044] Embodiment 3 The preparation of the compound extract of Teng japonica of the present invention
[0045] prescription:
[0046] Black bone vine 50g, Gentiana 20g, Yuanhu 20g
[0047] Preparation:
[0048] (1) Weigh the raw material drug by weight, ultrasonically extract it with 50% v / v ethanol, combine the extracts, recover the ethanol until it has no alcohol smell, and obtain the crude extract;
[0049] (2) Take the crude extract, put it on a D101 macroporous resin column, first elute with water to remove impurities (20 times column volume), and then use 20 times column volume 20%v / v, 40%v / v ethanol to elute successively , collected 40% ethanol eluate, concentrated under reduced pressure, and freeze-dried to obtain the compound extract of S.
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