Cascade mass spectrometry method performed in ion trap mass analyzer

Abstract

The invention belongs to the technical field of mass spectrometry and particularly relates to a cascade mass spectrometry method performed in an ion trap mass analyzer. The cascade mass spectrometry method performed in the ion trap mass analyzer specifically comprises three stages of ion selective segregation, collision induction dissociation and mass scanning and analyzing. According to the cascade mass spectrometry method performed in the ion trap mass analyzer, in the stage of collision induction dissociation, by changing the period of a radio frequency signal, namely changing the frequency of radio-frequency voltage loaded on an ion trap, parent ions with a certain mass-to-charge ratio undergo resonance excitation so as to obtain energy. The high-energy ions which undergo resonance excitation collide with neutral molecules in the ion trap and are dissociated, outcome ions are generated, and the cascade mass spectrometry is achieved. The cascade mass spectrometry method performed in the ion trap mass analyzer has the advantages that only arrangement of software can be used for changing the scanning period of the stage of collision induction dissociation in order to achieve collision induction dissociation, and thus, an experiment device and a method of cascade mass spectrometry can be obviously simplified.

Description

technical field [0001] The invention belongs to the technical field of mass spectrometry, and in particular relates to a method for realizing tandem mass spectrometry in an ion trap mass analyzer. Background technique [0002] As a powerful analytical technique, mass spectrometry can achieve qualitative and quantitative analysis of compounds, and is widely used in pharmaceutical analysis, environmental monitoring, national security, forensic science, proteomics and other fields. As we all know, mass spectrometry can characterize and analyze the structure of compounds through tandem mass spectrometry (Tandem MS) analysis. The analysis process of tandem mass spectrometry is as follows: the first stage is isolation. For the ions in the sample to be analyzed, ions with a specific mass-to-charge ratio (m / z) are selected to isolate them, and the isolated ions become parent ions. (parent ion); the second stage is Collision Induced Dissociation (CID). The parent ion collides with n...

AI Technical Summary

Problems solved by technology

However, this method requires an additional DC power supply to provide DC voltage. The dipole DC application method is realized through the modification of the circuit. At the same time, the dipole DC voltage has timing changes and requires precise control, so the hardware part of the instrument Requirements are bound to become more stringent and complex

Images