Application of radix paeoniae alba extract

The technology of extract and Radix Paeoniae Alba is applied in the application field of Radix Paeoniae Alba in the control of botrytis cinerea of ​​strawberry, which can solve the problems of rare reports on the research on the inhibitory effect of plant pathogens, and achieves safety and environmental protection for humans and animals, convenient operation, extraction simple method effect

Inactive Publication Date: 2013-11-20
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous research results show that the extract of Radix Paeoniae Alba has a certain inhibitory effect on animal pathogenic bacteria, but there are few reports on the inhibitory effect on plant pathogenic bacteria.

Method used

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  • Application of radix paeoniae alba extract
  • Application of radix paeoniae alba extract
  • Application of radix paeoniae alba extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The roots of Radix Paeoniae Alba were washed, dried in the sun, and pulverized into 95-mesh fine powder with a universal grinder at a speed of 6500 r / min to obtain Radix Paeoniae Alba powder. Take 10g of Radix Paeoniae Alba powder and put it into a conical flask, extract the Radix Paeoniae Alba powder with analytical pure acetone, add acetone in 3 times, add 30mL each time, put the conical flask on a shaker at 20°C and shake at 100r / min 60h, filter, collect the filtrate, combine the filtrate for 3 times, put the filtrate into a rotary concentration evaporator, under the conditions of 0.08MPa air pressure, water bath temperature of 45°C and condensation temperature of 4°C, evaporate and concentrate at 110r / min to a paste, to obtain Yellowish Paeoniae Alba Extract.

Embodiment 2

[0023] Take Radix Paeoniae Alba, wash it, dry it in the sun, and grind it into a fine powder of 105 mesh with a universal grinder at a speed of 7500 r / min to obtain Radix Paeoniae Alba powder. Take 10g of Radix Paeoniae Alba powder and put it into a conical flask, extract the Radix Paeoniae Alba powder with analytically pure acetone, add acetone in 3 times, add 40mL each time, put the conical flask on a shaker at 25°C and shake at 120r / min 54h, filter, collect the filtrate, combine the filtrate for 3 times, put the filtrate into a rotary concentration evaporator, and evaporate and concentrate at 130r / min to a paste under the conditions of 0.09MPa air pressure, water bath temperature of 50°C and condensation temperature of 5°C to obtain Yellowish Paeoniae Alba Extract.

Embodiment 3

[0025] Take Radix Paeoniae Alba, wash it, dry it in the sun, and grind it into a fine powder of 115 mesh with a universal grinder at a speed of 8500 r / min to obtain Radix Paeoniae Alba powder. Take 10g of Radix Paeoniae Alba powder and put it into a conical flask, extract the Radix Paeoniae Alba powder with analytical pure acetone, add acetone in 3 times, add 50mL each time, put the conical flask on a shaker at 30°C and shake at 150r / min 48h, filter, collect the filtrate, combine the filtrate for 3 times, put the filtrate into a rotary concentration evaporator, and evaporate and concentrate at 150r / min to a paste under the conditions of 0.1MPa air pressure, water bath temperature of 55°C and condensation temperature of 6°C to obtain Yellowish Paeoniae Alba Extract.

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Abstract

The invention relates to an application of a radix paeoniae alba extract for controlling grey mould fruit rot of strawberry. The extract has a remarkable inhibiting effect and a certain in-vitro control efficiency to grey mould fruit rot of strawberry by way of inhibiting molecular spore germination, sclerotium germination and the like, and can be further developed to a novel plant source bactericide.

Description

technical field [0001] The invention belongs to the field of biotechnology, and mainly relates to the application of Radix Paeoniae Alba extract, in particular to the application of Radix Paeoniae Alba in controlling gray mold of strawberries. Background technique [0002] Strawberry not only has high nutritional value, but also can strengthen gums, freshen breath, moisturize throat, beautify and enhance resistance. In recent years, with the improvement of living standards, people's consumption of strawberries has also increased year by year, which has brought vigor and vitality to the development of the strawberry industry. Therefore, the production area is relatively fixed, and repeated planting of strawberries in one place for many years is easy to accumulate pathogenic bacteria, which often leads to the occurrence and aggravation of strawberry diseases. Among them, strawberry gray mold is one of the main diseases that endanger strawberry production. [0003] Strawberry ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/32A01P3/00
Inventor 张亚廖晓兰黄璜刘双清王凌宇阳盼刘芳
Owner HUNAN AGRICULTURAL UNIV
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