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Upland cotton protein GhMADS22, and coding gene and application thereof

A technology encoding genes and proteins, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problem of insufficient early maturity of short-season cotton, and achieve the effect of increasing cotton yield

Inactive Publication Date: 2015-02-11
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current short-season cotton has the problem of insufficient early maturity

Method used

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  • Upland cotton protein GhMADS22, and coding gene and application thereof
  • Upland cotton protein GhMADS22, and coding gene and application thereof
  • Upland cotton protein GhMADS22, and coding gene and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] 1. Extraction of RNA

[0066] Follow the instructions of the kit, and the specific steps are as follows:

[0067] Before operation, prepare lysate SL containing 5% mercaptoethanol at a final concentration, for example, add 50 μl β-mercaptoethanol to 950 μl SL.

[0068] 1). Homogenization treatment:

[0069] 100 mg of China Cotton Research Institute 36 (CCRI36) plant leaves were quickly ground into powder in liquid nitrogen, and 500 μl of the prepared lysate SL was added (please check whether β-mercaptoethanol has been added before use), vortexed vigorously to mix, and kept at room temperature Leave it for 5min.

[0070] 2). Transfer all the solution to the filter column CS (the filter column CS is placed in the collection tube), centrifuge at 12,000rpm (~13,400×g) for 2 minutes, carefully pipette the supernatant in the collection tube to a new 1.5ml RNA-free For enzyme (RNase-free) centrifuge tubes, the tip should try to avoid contact with the cell debris in the coll...

Embodiment 2

[0101] 1. Analysis of spatiotemporal expression pattern of cotton gene GhMADS22

[0102] In order to study the expression pattern of GhMADS22 gene, qRT-PCR was used to detect the expression of GhMADS22 in different developmental stages of CMI 36 terminal buds and in different tissues.

[0103] Using the 10-fold diluted terminal buds and cDNAs of different tissues of Zhongmian Institute 36 as templates and ACTIN as an internal reference, the fluorescence relative quantitative analysis of the gene GhMADS22 was carried out.

[0104] The primer sequences used to amplify the gene GhMADS22 are:

[0105] Forward primer q22F: 5'-AGGAAATGACCCATCAGCCAC-3'

[0106] Reverse primer q22R: 5'-GCTGCACTAGGATTACCCCTCTTC-3'

[0107] ACTIN internal reference primer sequence is:

[0108] Forward primer: 5'-ATCCTCCGTCTTGACCTTG-3'

[0109]Reverse primer: 5'-TGTCCGTCAGGCAACTCAT-3'

[0110] The specific operation steps are as follows:

[0111] 1) Design fluorescent quantitative primers with Olig...

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Abstract

The invention discloses an upland cotton GhMADS22 protein, and a coding gene and application thereof. The protein GhMADS22 has one of the following amino acid residue sequences: 1) amino acid residue sequence disclosed as SEQ ID No.2 in the sequence table; and 2) 1) derived protein related to plant photosynthetic efficiency, which is subjected to substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid residue sequence disclosed as SEQ ID No.2 in the sequence table. The protein and coding gene thereof can be used for cultivating cotton with advanced reproductive growth, higher planting density or increased number of secondary bolls, and enhancing the cotton yield, thereby laying foundation for cultivation of transgenic plants.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an upland cotton protein, its coding gene and its application. Background technique [0002] Cotton is one of the most important economic crops in our country and plays an important role in national production. However, the development of cotton is limited to a certain extent due to the land competition between grain and cotton. Breeding and promotion of short-season cotton varieties can alleviate the conflict between grain and cotton land, and is an effective way to achieve double harvests of grain and cotton. However, the current short-season cotton has the problem of insufficient prematurity. [0003] As one of the important traits of cotton, early maturity has become an important breeding goal of upland cotton. Studies have shown that flowering sooner or later is closely related to the early maturity of cotton. Therefore, the flowering-related genes were cloned, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N15/11A01H5/00
Inventor 喻树迅张文香范术丽宋美珍庞朝友魏恒玲
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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