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Ficus tikoua rapid propagation method

A ground loquat and fast technology, which is applied in the field of tissue culture and rapid propagation of ground loquat, can solve the problems of no loquat tissue culture research and patent application, and achieve the effects of effective protection, development and utilization, low production cost, and fast seedling emergence.

Inactive Publication Date: 2014-08-06
INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to inquiries, there are currently no reports of loquat tissue culture research and patent applications at home and abroad

Method used

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  • Ficus tikoua rapid propagation method
  • Ficus tikoua rapid propagation method
  • Ficus tikoua rapid propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, the loquat aseptic seedling of tissue culture rapid propagation ground

[0030] 1. Culture medium preparation and sterilization

[0031] Primary medium: MS+6-BA0.8mg / L+IAA0.3mg / L;

[0032] Subculture medium: N6 macroelement + MS trace element + MS organic component + MS iron salt + 6-BA1.0mg / L + KT0.5mg / L + IAA0.2mg / L + paclobutrazol (CCC) 1.0mg / L;

[0033] Rooting medium: MS+IAA0.2mg / L+NAA0.5mg / L+KT0.5mg / L+paclobutrazol (CCC) 0.5mg / L;

[0034] The above medium was sterilized at 121°C for 20 minutes.

[0035] 2. Primary culture

[0036] Take the terminal buds or axillary buds with stems of loquat (Ficus tikoua) (the total length of stems and buds is 2-3cm), first wash them with washing powder, wash them with sterile water 5-7 times on a sterile operating table, and use 70% Soak in alcohol for 1 minute, then sterilize in 0.1% mercuric chloride solution for 12 to 18 minutes, rinse with sterile water for 5 to 6 times, transfer the terminal buds or axillar...

Embodiment 2

[0047] Embodiment 2, the aseptic seedling of loquat in tissue culture rapid propagation ground

[0048] Media Preparation and Sterilization

[0049] Primary medium: MS+6-BA1.0mg / L+IAA0.5mg / L;

[0050] Subculture medium: N6 massive + MS trace + MS organic + MS iron salt + 6-BA1.0mg / L + KT0.3mg / L + IAA0.2mg / L + paclobutrazol (CCC) 0.5mg / L;

[0051] Rooting medium: MS+IAA0.3mg / L+NAA0.3mg / L+KT0.5mg / L+paclobutrazol (CCC) 0.5mg / L.

[0052] Sterilize the above medium at 121°C for 20 minutes;

[0053] The terminal buds or stems of loquat (Ficus tikoua) are taken, and the basic operation method is the same as that in Example 1. The effect of multiplication and rooting is slightly worse than that in Example 1, but it can also be achieved very well.

Embodiment 3

[0054] Embodiment 3, tissue culture rapid propagation ground loquat aseptic seedling

[0055] Primary medium: MS+6-BA0.5-1.5mg / L+IAA0.1-0.8mg / L

[0056] The subculture medium is: macroelements of N6 medium + trace elements of MS medium + organic components of MS medium + iron salt of MS medium + 6-BA1.0-2.5mg / L + KT0.2- 0.5mg / L+IAA0.1-0.8mg / L+paclobutrazol 0.5-2.0mg / L.

[0057] The rooting culture medium is: MS+IAA0.1-0.8mg / L+NAA0.1-0.8mg / L+KT0.5-1.0mg / L+paclobutrazol 0.2-1.0mg / L.

[0058] Within the concentration ranges of the above-mentioned several culture media, the operation steps in Example 1 can be followed, and satisfactory results can basically be achieved.

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Abstract

The invention discloses a ficus tikoua rapid propagation method. According to the method, ficus tikoua terminal buds or axillary buds with stem sections are cultured in an initial medium, such that aseptic seedlings are obtained; clustered buds are induced on a proliferation medium; and rooting is carried out on a rooting medium. When ficus tikoua tissue culture is carried out with the set of technical methods provided by the invention, the period is short, propagation coefficient is high, cost is low, and high factory production capacity is provided. When propagation is carried out with the method provided by the invention, ficus tikoua can be effectively protected, developed, and utilized.

Description

technical field [0001] The invention belongs to the technical field of rapid propagation of loquat, in particular to a method for rapid propagation of loquat (Ficus tikoua) through tissue culture. Background technique [0002] Ground loquat (Ficus tikoua) belongs to the genus Moraceae, also known as sweet potato, sweet potato vine, ground gall, and ground pomegranate. A perennial deciduous creeping shrub with latex in the whole plant, produced in Guangxi, Guizhou, Hunan and other places, and used as a traditional medicinal material of the Miao people in Guizhou and Xiangxi. Pharmacological studies on this genus at home and abroad have shown that this genus has hypoglycemic, smooth muscle relaxation, anti-tumor, and antibacterial effects. Ground loquat can also be used as fruit, delicious and sweet, bright in color, its fruit is like figure 1 shown. The ground loquat can also be used as a hanging bonsai for fruit viewing and leaf viewing. The flexible and emerald green ste...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 夏新界黄丽芳
Owner INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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