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Cycloamylose preparation method based on starch debranching method

A technology of macrocyclodextrin and starch, which is applied in the field of preparation of macrocyclodextrin

Active Publication Date: 2013-10-16
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] As we all know, starch is composed of two types of amylose and amylopectin. The ratio of amylose and amylopectin in natural starch is about 15~28% to 72~ 85%, since the optimal substrate of 4-α-glycosyltransferase is amylose, the amount of macrocyclodextrin prepared by acting on amylopectin is very small, so that the natural starch containing a large amount of amylopectin cannot For industrial production of macrocyclodextrins
At present, the production method of macrocyclodextrin is usually based on potato amylose with high amylose content as the substrate, and is produced by the action of 4-α-glycosyltransferase. Although this method can obtain higher conversion However, due to the high cost of amylose preparation and high price, its large-scale production and industrial application are greatly limited.

Method used

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  • Cycloamylose preparation method based on starch debranching method
  • Cycloamylose preparation method based on starch debranching method
  • Cycloamylose preparation method based on starch debranching method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Production of macrocyclodextrin by debranching method from common corn starch

[0031] Concrete preparation steps are as follows:

[0032] (1) Debranching pretreatment of corn starch and preparation of 1wt% starch solution: Weigh 10 mg of ordinary corn starch (purchased from Hangzhou Prostar Starch Co., Ltd.), add 1 ml of 90% dimethyl sulfoxide, and place in boiling water bath for 1 h Magnetically stir overnight at room temperature, then add 6 times the volume of absolute ethanol, centrifuge at 5000rpm for 10min, discard the supernatant, add 1ml of preheated sodium acetate buffer (50mM, pH 3.5) to the resulting precipitate, and boil for 30min. Obtain 1wt% common corn starch solution.

[0033] (2) Debranching of common corn starch: Add 7.5U / g isoamylase (purchased from Megazyme, Ireland) to the 1wt% common corn starch solution prepared in step (1), and debranch at 40°C and pH 3.5. Branch for 12 hours, then inactivate the enzyme in a boiling water bath for ...

Embodiment 2

[0040] Example 2 Production of macrocyclodextrin by debranching method of common tapioca starch

[0041] Concrete preparation steps are as follows:

[0042] (1) Debranching pretreatment of tapioca starch and preparation of 1wt% starch solution: Weigh 10 mg of ordinary tapioca starch (purchased from Hangzhou Prostar Starch Co., Ltd.), add 1 ml of 90% dimethyl sulfoxide, and place in a boiling water bath for 1 h Magnetically stir overnight at room temperature, then add 6 times the volume of absolute ethanol, centrifuge at 5000rpm for 10min, discard the supernatant, add 1ml of preheated sodium acetate buffer (50mM, pH 3.5) to the resulting precipitate, and boil for 30min. Obtain 1wt% common tapioca starch solution.

[0043] (2) Debranching of ordinary tapioca starch: add 5U / g isoamylase (same as above) to the 1wt% ordinary tapioca starch solution prepared in step (1), debranch at 40°C and pH 3.5 for 12 hours, then boil water Bath for 10min to inactivate the enzyme.

[0044]...

Embodiment 3

[0050] Example 3 Production of Macrocyclodextrin by Debranching High Amylose Corn Starch

[0051] Concrete preparation steps are as follows:

[0052] (1) Debranching pretreatment of high-amylose corn starch and preparation of 1wt% starch solution: Weigh 10 mg of high-amylose corn starch (purchased from Hangzhou Prostar Starch Co., Ltd.), add 1 ml of 90% dimethyl sulfoxide, and bathe in boiling water for 1 h Magnetically stir overnight at room temperature, then add 6 times the volume of absolute ethanol, centrifuge at 5000rpm for 10min, discard the supernatant, add 1ml of preheated sodium acetate buffer (50mM, pH 3.5) to the resulting precipitate, and boil for 30min Promptly get 1wt% high amylose corn starch solution.

[0053] (2) Debranching of high-amylose corn starch: Add 10 U / g isoamylase (same as above) to the 1wt% high-amylose corn starch solution prepared in step (1), debranch at 40°C and pH 3.5 for 12 hours, then boil water Bath for 10min to inactivate the enzyme. ...

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Abstract

The invention discloses a cycloamylose preparation method based on starch debranching method. Starch is taken as an enzyme reaction substrate, and subject to starch debranching, 4-alpha-glycosyl transferase function, maltogenic amylase treatment, ethanol precipitation and freeze-drying to obtain a cycloamylose finish product; the starch is natural starch from different sources and comprises common corn starch and common cassava starch. According to the invention, the low-price natural starch is directly adopted as the substrate to prepare the pure cycloamylose with high conversion rate and high purity, the process is simple and economy and high efficiency are achieved.

Description

technical field [0001] The invention relates to a preparation method of macrocyclodextrin, in particular to a preparation method of macrocyclodextrin based on starch debranching method. It belongs to the technical field of food processing. Background technique [0002] Macrocyclodextrin is a general term for a class of cyclic maltolans composed of 9 or more glucose residues connected by α-1, 4 glycosidic bonds. It has the characteristics of good water solubility, low viscosity, and difficult retrogradation. The guest molecules form clathrates to protect the guest molecules and improve the solubility of the guest molecules. Therefore, it has great application potential in the fields of food, medicine and chemical industry. [0003] As we all know, starch is composed of two types of amylose and amylopectin. The ratio of amylose and amylopectin in natural starch is about 15-28% to 72-85%. The substrate is amylose, and the amount of macrocyclodextrin prepared by acting on amyl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/20C12P19/18C12P19/16C12P19/04
Inventor 金征宇许燕周星王金鹏徐学明
Owner JIANGNAN UNIV
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