Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

On-line pre-separating and separating analysis method for whole protein and liquid chromatogram system thereof

A technology of separation analysis and pre-separation, which is applied in the direction of material separation, analysis materials, measuring devices, etc. It can solve the problems of slow analysis speed, low sensitivity, time-consuming loss, etc., and achieves increased speed, maximized separation resolution, and simple structure Effect

Inactive Publication Date: 2013-09-25
TONGJI UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the sample loss in the desalting process is very serious and time-consuming, the analysis speed is slow and the sensitivity is low when ion exchange chromatography is used for pre-separation
The eluent of size exclusion chromatography is compatible with liquid chromatography-mass spectrometry analysis, can be used online, and the analysis speed is fast, but its resolution is often very low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • On-line pre-separating and separating analysis method for whole protein and liquid chromatogram system thereof
  • On-line pre-separating and separating analysis method for whole protein and liquid chromatogram system thereof
  • On-line pre-separating and separating analysis method for whole protein and liquid chromatogram system thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0053] The working process of the liquid chromatography system will be described below by taking the separation and analysis of the whole proteome of Escherichia coli as an example.

[0054] Step 1, sample injection: in the injection valve, the protein sample is injected into the sample loop by the syringe, such as figure 1 shown;

[0055] Step 2, pre-separation: Mobile phase A (100% water + 0.1% formic acid) carries the sample in the sample loop through T1, T2, T3, T4 and T5 trapping columns in sequence, such as figure 2 shown;

[0056] Step 3, C1 separation: The sample trapped on the T1 trapping column (filled with C1 stationary phase) is separated by the A1 analytical column (filled with C1 stationary phase) and detected by mass spectrometry. The connection positions of each chromatographic valve are as follows: image 3 Shown; The chromatogram obtained by analyzing the protein trapped on the T1 trapping column (packed with C1 stationary phase) by A1 analytical column (p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an on-line pre-separating and separating analysis method for a whole protein and a liquid chromatogram system thereof. By utilizing orderly proportionately increased characteristic of hydrophobicity of Cn capture column, proteins in a protein mixture are orderly gradingly captured from large to small to implement pre-separation; and the proteins on the capture column are orderly matched by relative Cn analytical columns to implement secondary separation, and the analytical column is used together with the mass spectrum for analysis. The orderly proportionately increased capture columns Cn (n=1-18 or partial combination thereof, such as 1, 2, 4, 8 and 18) are used for on-line pre-separating samples, which greatly raises a pre-separation speed, sensitivity and resolution; and the separation columns and the capture column are pairwise matched (such as C4 capture and C4 separation column are matched), which have consistent hydrophobicity, thereby implementing maximization of separation resolution.

Description

technical field [0001] The invention relates to a technology and method for on-line fractionation, pre-separation and separation analysis of whole proteins. Background technique [0002] In today's post-genome era, the entire genome sequences of many species have been or are being determined, which makes comprehensive and in-depth research on the proteome possible. Since most of the biological functions are ultimately transmitted and realized through proteins, the research on proteins will promote the development of biology and medicine more directly and rapidly on the basis of gene research. The technology based on high performance liquid chromatography-mass spectrometry can quickly and sensitively analyze and identify proteins and their mixtures, and is the frontier and preferred research method for protein research. In the past ten years, mass spectrometry-based protein analysis and identification technology has been developed rapidly, and it has been able to perform com...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/06
Inventor 田志新
Owner TONGJI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com