Enzyme feed additive
A technology for feed additives and culture strains, applied in enzyme feed additives, enzymes in the field of feed applications, can solve the problems of cellulase production and application limitations, high production costs, poor enzyme stability, etc., to achieve the improvement of cultured food Safe, beneficial to environmental protection, and the effect of enhancing disease resistance
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[0018] 1. Cultivation of Chaetomyces thermophilic-grade slant:
[0019] The thermophilic Chaetomium was inoculated on the primary slant medium by conventional methods, and cultivated at 30° C. for 24 hours to obtain a single colony of the thermophilic Chaetomium; the medium was a mixture prepared in the following ratio ( g / L): beef peptone: 5.0, yeast extract: 15.0, glucose: 3.0, MgSO 4 ·7H 2 O: 0.5, K 2 HPO 4 : 1.0, MnSO 4 : 20ppm, CaCO 3 : 5.0, agar 20, pH 7.0;
[0020] 2. Secondary liquid culture of Chaetomium thermophiles
[0021] Inoculate the single colony of the first-level thermophilic Chaetomium cultured in step 1 into the second-level culture medium by a conventional method, with a liquid volume of 20ml / 500ml, and cultivate it for 25 hours at 35°C and 180rpm / min to obtain a second-level thermophile Chaetomium seed liquid; the medium is a mixed solution (g / L) prepared in the following proportions: soybean peptone: 5.0, yeast extract: 5.0, glucose: 3.0, MgSO 4 ...
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