Method for producing ethanol by fermenting steam explosion quercus acutissima carr
An acorn shell, ethanol technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve problems such as inability to use well, pollute the environment, energy, waste, etc., to achieve sustainable development, The effect of protecting the ecological environment and reducing costs
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Embodiment 1
[0019] The acorns (collected from Chuzhou, Anhui) were air-dried and shelled, and the acorn shells were dried at 105°C for later use. The acorn shells were pretreated with a steam explosion machine, the volume ratio of the mass of the acorn shells to water was 1:20 (g: ml), the explosion pressure was 1.0 MPa, and the duration was 6 minutes. After treatment, wash the raw material with distilled water until neutral, dry at 105°C, pulverize with a pulverizer, and pass through a 40-60 mesh sieve.
[0020] Take a certain amount of blasting material powder, add water according to the mass volume ratio of 1:20 (g: ml), at 60°C, with a power of 300W, ultrasonic cleaning for 20min, then filter, and dry at 105°C to obtain tannin-removed acorns shell powder.
[0021] Weigh the tannin-removed acorn shell powder obtained in the above steps, add 0.1M, pH 4.8 citric acid buffer solution, the ratio of solid to liquid is 1:10 (g: ml), preheat in a water bath at 50°C for 20 minutes, and then f...
Embodiment 2
[0023] The dried acorn shells were pretreated with a steam explosion machine, the volume ratio of the mass of acorn shells to water was 1:10 (g: ml), the burst pressure was 2.0 MPa, and the duration was 10 minutes. After treatment, wash the raw materials with distilled water until neutral, dry at 105°C, pulverize with a pulverizer, and pass through a 40-60 mesh sieve to obtain blasting material powder for use.
[0024] Take a certain amount of blasting material powder, add water according to the mass volume ratio of 1:25 (g: ml), at 60°C, with a power of 300W, ultrasonic cleaning for 40min, then filter, and dry at 105°C to obtain tannin-removed acorns shell powder.
[0025] Weigh the de-tannin acorn shell powder obtained in the above steps and add 0.1M, pH 4.8 citric acid buffer solution, the mass volume ratio of the de-tannin acorn shell powder and citric acid buffer solution is 1:10 (g:ml) , Preheat in a water bath at 50°C for 20 minutes, then add cellulase according to...
Embodiment 3
[0028] The dried acorn shells were pretreated with a steam explosion machine. The volume ratio of the mass of acorn shells to water was 1:10 (g: ml), and the burst pressure was 1.5 MPa for 8 minutes. After treatment, wash the raw materials with distilled water until neutral, dry at 105°C, pulverize with a pulverizer, and pass through a 40-60 mesh sieve to obtain blasting material powder for use.
[0029] Take a certain amount of blasting material powder, add water according to the mass volume ratio of 1:20 (g: ml), wash at 50°C with a power of 300W for 30min, then filter and dry at 105°C to obtain tannin-removed acorn shells powder.
[0030] Weigh the de-tannin acorn shell powder obtained in the above steps and add 0.1M, pH 4.8 citric acid buffer solution, the mass volume ratio of the de-tannin acorn shell powder and citric acid buffer solution is 1:10 (g:ml) , Preheat in a water bath at 50°C for 20 minutes, then add cellulase according to the enzyme amount of 20FPU / g pow...
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