Method and application for increasing oligomerization wood sugar content in black tea

A technology of xylooligosaccharides and black tea, which is applied in the field of microbial fermentation, can solve problems such as difficult to achieve, achieve the effect of improving quality, stabilizing quality, and overcoming quality instability

Inactive Publication Date: 2013-09-18
湖南惟楚福瑞达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

To achieve an effective intake, people need to drink as much as

Method used

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  • Method and application for increasing oligomerization wood sugar content in black tea
  • Method and application for increasing oligomerization wood sugar content in black tea
  • Method and application for increasing oligomerization wood sugar content in black tea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation of spore liquid of thermophilic cotton wool

[0026] Spread the thermophilic cotton wool on the PDA agar medium plate, culture at 50°C for 6-8 days, add an appropriate amount of sterile water to the plate, scrape off the spores with a sterile scraper, suspend in sterile water, and then suspend the The solution was poured into a conical flask containing glass beads, shaken at 200r / min for 1h to make a spore suspension, counted on a cell counting board, and the concentration of spores was controlled at 1×10 6 ~5×10 6 individual / mL.

[0027] Preparation of spore liquid of Cystis coronis

[0028] Coronoidis spread on the PDA agar medium plate, cultured at 30°C for 6-8 days, add an appropriate amount of sterile water to the plate, scrape off the spores with a sterile scraper, suspend them in sterile water, and then The suspension was poured into a conical flask containing glass beads, shaken on a shaker at 200r / min for 1h to make a spore suspension, counted on...

Embodiment 2

[0033] The preparation of the thermophilic cotton wool spore liquid and the Saccharomyces coronis spore liquid is the same as that in Example 1.

[0034] Specific implementation steps: Spray 300g of Maocha with water evenly until the water content reaches 20%, put the tea in a pressure-resistant sterilizer, heat up to 100°C, steam for 10 minutes, take out the Maocha, and evenly spray the diluent of thermophilic cotton wool spores , control the water content of the tea leaves to 26%, place them in an incubator with a temperature of 50°C and a relative humidity of 75%, and cultivate them for 2 days. Put the cultivated tea leaves in a pressure-resistant sterilizer, raise the temperature to 100°C, steam for 5 minutes, take out the raw tea, evenly spray the spore liquid of S. coronoidis, control the water content of the tea leaves to 27%, press the tea bricks, and place Cultivate in an incubator with a temperature of 28-30°C and a relative humidity of 75% for 5-7 days. After the cu...

Embodiment 3

[0038] The preparation of the thermophilic cotton wool spore liquid and the Saccharomyces coronis spore liquid is the same as that in Example 1.

[0039]Specific implementation steps: Spray 300g of Maocha with water evenly until the water content reaches 20%, put the tea in a pressure-resistant sterilizer, heat up to 100°C, steam for 10 minutes, take out the Maocha, and evenly spray the diluent of thermophilic cotton wool spores , control the water content of the tea leaves to 28%, place them in an incubator with a temperature of 50°C and a relative humidity of 75%, and cultivate them for 3 days. Put the tea after cultivation in a pressure-resistant sterilizer, raise the temperature to 100°C, steam for 5 minutes, take out the raw tea, evenly spray the spore liquid of Saccharomyces coronis, control the water content of the tea to 26%, press the tea brick, and place Cultivate in an incubator with a temperature of 28-30°C and a relative humidity of 75% for 5-7 days. After the cul...

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Abstract

The invention provides a method for increasing the oligomerization wood sugar content in black tea aiming at the disadvantages that the oligomerization wood sugar content in the black tea at present is low and the effect is inadequately obvious. The method is characterized in that heat-resistant xylanase is produced in the black tea fermentation process through inoculating a xylanase producing strain, namely Thermomyces lanuginosus, and thus oligomerization wood sugar produced by xylan in the black tea is degraded; detection results prove that compared with the traditional black tea, the oligomerization wood sugar content in the black tea prepared by using the method is increased by 20-40 times; the animal experiment results prove that bifidobactirium proliferation in the intestinal tracts of white rats fed by the black tea is obvious and is improved by about 8% compared with that in the intestinal tracts of the white rats fed by the traditional feeding black tea, and the growth of enteric bacilli, enterococcus and the like can be inhibited to different degrees.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to black tea processing technology. Background technique [0002] Black tea is a kind of fermented tea, which can be made into loose tea or pressed into Fu bricks, etc. It is mainly sold in the minority areas in the northwest frontier of China. It is resistant to storage, easy to transport long distances, and rich in nutrients. Mysterious Tea on the Road", "Tea of ​​Life of Minorities in Northwest China". The traditional processing process of dark tea actually uses the tea itself and microorganisms in the air to undergo a series of enzymatic reactions in a humid and hot environment, catalyzing various components in the tea, and producing new oxidation, polymerization, degradation, and transformation reactions. The quality of tea is driven by the interlacing of physical and chemical power, and the special quality change is completed, forming a specific quality of so...

Claims

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Application Information

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IPC IPC(8): A23F3/10
Inventor 彭呈悦凌霄李征
Owner 湖南惟楚福瑞达生物科技有限公司
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