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Efficient bletilla propagation method using pseudobulbs to induce pseudobulbs

A technology of pseudobulb and bletilla striata, which is applied in the field of high-efficiency breeding of bletilla striata, can solve the problems of restricting the popularization and application of tissue cultured seedlings and high prices, and achieve the goal of providing seedling guarantee and technical support, low production cost, and robust tissue cultured seedlings Effect

Inactive Publication Date: 2013-09-18
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The technology is divided into the steps of primary induction, subculture multiplication, rooting culture and transplanting, etc. The reproduction coefficient is mainly reflected in the technical link of subgeneration multiplication. At present, the reproduction coefficient of Bletilla striata rapid propagation technology is 2~5, and its composition is calculated based on this. The cost and price of cultivating seedlings remain high, which has become the main factor restricting the popularization and application of tissue culture seedlings

Method used

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  • Efficient bletilla propagation method using pseudobulbs to induce pseudobulbs

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Embodiment 1

[0022] A high-efficiency propagation method of Bletilla striata using pseudobulbs to induce pseudobulbs. The existing method is used to perform the steps of primary induction (seed germination) and subsequent multiplication to obtain clustered buds. The method of inducing pseudobulbs with pseudobulbs is used in the stage of rooting and strong seedlings, including the following step:

[0023] (1) On the ultra-clean workbench, the clustered buds of Bletilla striata are cut into single buds and inserted into the rooting medium, cultivated for 30 days, the base of the bletilla striata buds expand to form pseudobulbs, and have roots; the rooting medium is: 1 / 2MS+6-BA 0.2 mg / L+IBA 0.5 mg / L+IAA 1.0 mg / L+0.1% activated carbon+15% potato+sucrose 20 g / L+agar 5 g / L, the pH value is 6.5;

[0024] (2) On the ultra-clean workbench, add the sterilized inducer to the tissue cultured seedlings with pseudobulbs in step (1), add 10-20 ml to each bottle, and buds will grow on the pseudobulbs for ...

Embodiment 2

[0028] A high-efficiency propagation method of Bletilla striata using pseudobulbs to induce pseudobulbs. The existing method is used to perform the steps of primary induction (seed germination) and subsequent multiplication to obtain clustered buds. The method of inducing pseudobulbs with pseudobulbs is used in the stage of rooting and strong seedlings, including the following step:

[0029] (1) On the ultra-clean workbench, the clustered buds of Bletilla striata are cut into single buds and inserted into the rooting medium, cultivated for 30 days, the base of the bletilla striata buds expand to form pseudobulbs, and have roots; the rooting medium is: 1 / 2MS+6-BA 0.2 mg / L+IBA 0.5 mg / L+IAA 1.0 mg / L+0.1% activated carbon+15% potato+sucrose 20 g / L+agar 5 g / L, the pH value is 6.5;

[0030] (2) On the ultra-clean workbench, add the sterilized inducer to the tissue culture seedlings with pseudobulbs in step (1), add 10-20 ml to each bottle, grow buds on the pseudobulbs for 12 days, a...

Embodiment 3

[0034] A high-efficiency propagation method of Bletilla striata using pseudobulbs to induce pseudobulbs. The existing method is used to perform the steps of primary induction (seed germination) and subsequent multiplication to obtain clustered buds. The method of inducing pseudobulbs with pseudobulbs is used in the stage of rooting and strong seedlings, including the following step:

[0035](1) On the ultra-clean workbench, the clustered buds of Bletilla striata are cut into single buds and inserted into the rooting medium, cultivated for 30 days, the base of the bletilla striata buds expand to form pseudobulbs, and have roots; the rooting medium is: 1 / 2MS+6-BA 0.2 mg / L+IBA 0.5 mg / L+IAA 1.0 mg / L+0.1% activated carbon+15% potato+sucrose 20 g / L+agar 5 g / L, the pH value is 6.5;

[0036] (2) On the ultra-clean workbench, add the sterilized inducer to the tissue culture seedlings with pseudobulbs in step (1), add 10-20 ml to each bottle, and grow buds on the pseudobulbs for 8 days,...

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Abstract

The invention discloses an efficient bletilla propagation method using pseudobulbs to induce pseudobulbs. The existing method is adopted and the steps of primary induction (seed germination) and mulitiplication are performed to obtain multiple shoots; different from the prior art, a method of inducing pseudobulbs with the pseudobulbs is adopted at the stage of taking root to grow a strong seedling. By utilizing the method, the propagation coefficient can be increased by 3-5 times on the previous basis; as the propagation coefficient is increased, the tissue cultured seedlings are strong and the root system is prosperous, so that the production cost of the tissue cultured seedlings of bletilla can be greatly reduced. Besides, the method is simple to operate and low in production cost; the method can be used for producing lots of good-quality seedlings in short time, and providing seeding guarantee and technical support for large-scale artificial cultivation of bletilla.

Description

technical field [0001] The invention relates to the technical field of plant asexual reproduction, in particular to a high-efficiency propagation method of bletilla striata using pseudobulbs to induce pseudobulbs. Background technique [0002] Bletilla striata ( Bletilla striata (Thunb.) Reichb.f.) also known as Baiji, ground screw, white chicken, knife-edge medicine, Lianjicao, is a perennial herb of Orchidaceae. Bletilla striata has been used as medicine for thousands of years in my country. Its pseudobulb has the effects of astringent and hemostasis, nourishing the lungs, reducing swelling and promoting muscle growth. It can be used externally to treat traumatic bleeding, pain, swelling, burns and sores. It can be used internally to treat hematemesis, lung disease, and cough. blood, chronic gastric ulcer and tumors etc. Modern studies have shown that the extract of Bletilla striata not only has no toxic side effects, but also has strong anti-oxidation and anti-aging effe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 石云平赵志国黄宁珍付传明唐凤鸾
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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