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Kelp germplasm separation method

A separation method, the technology of kelp, applied in the direction of plant cells, etc., can solve the problems of low separation efficiency and easy contamination of kelp germplasm separation, and achieve the effect of improving separation efficiency, avoiding pollution, and satisfying rapid growth

Active Publication Date: 2013-08-28
MARICULTURE INST OF SHANDONG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for separating germplasm of kelp, thereby solving the problem that germplasm separation of kelp is easily polluted and the separation efficiency is low, so as to make up for the deficiencies in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Collect kelp leaves with mature sporangia when the seawater temperature reaches 16°C in the introduced sea area;

[0018] (2) Transport it indoors, cut small pieces of 20×20 cm with a blade, then wipe the kelp leaves with mature sporangia with sterile gauze, dry and stimulate in the shade for 1 hour, wrap them with gauze and 200-mesh sieve silk and put them in a container In the container of disinfected seawater, control the water temperature at 10-12°C to let the spores disperse;

[0019] (3) When the density of zoospores in seawater under microscope inspection is 5-10 / 100X, put them into slides.

[0020] (4) Continue to cultivate the slides after the spores attach, control the light intensity at 800-1000lx, light for 12 hours a day, control the water temperature at 10-12°C, and form gametophytes after 8 days of cultivation.

[0021] (5) Scrape the gametocytes on the glass slide into the embryo culture dish, add sterilized seawater to dilute 10 times, microscopica...

Embodiment 2

[0025] (1) Collect kelp leaves with mature sporangia when the seawater temperature reaches 15°C in the introduced sea area;

[0026] (2) Transport it indoors, cut small pieces of 30×30 cm with a blade, then wash the kelp leaves with mature sporangia with sterile gauze dipped in sterile sea water, dry in the shade and stimulate for 80 minutes, wrap it in gauze, and then use 200 Wrap the silk with a mesh sieve and put it into a container filled with sterilized seawater, and control the water temperature at 8-10°C to let the spores disperse;

[0027] (3) When the density of zoospores in the seawater under microscope inspection is 10-20 / 100X, put it into the glass slide.

[0028] (4) The slides after the spores attached were placed in a temperature-controlled light incubator for cultivation, the light intensity was controlled at 900-1100lx, the light was 12 hours a day, and the water temperature was controlled at 10-12°C. After 7 days of cultivation, gametophytes were formed.

[...

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PUM

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Abstract

The invention relates to a kelp germplasm separation method. According to the kelp germplasm separation method, when kelp zoospores are diffused, kelp is inside and outside wrapped by medicine, gauze and bolting-silk, so that kelp mucus and impurities can be effectively prevented from influencing water quality; a pipettor separation gametophyte can be used for effectively improving the separation efficiency and avoiding pollution; and a culture medium is improved, so that the rapid growth requirement of the kelp zoospores is satisfied, and the pollution can be effectively avoided. Therefore, the kelp germplasm separation method is safe and high-efficiency.

Description

technical field [0001] The invention belongs to the technical field of seaweed germplasm separation, and in particular relates to a seaweed germplasm separation method. Background technique [0002] Kelp is a large brown algae with high economic value and has become the pillar industry of aquaculture in my country. However, because my country is not the country of origin of kelp, there is only one original species of kelp, and its biodiversity is low. After long-term cultivation, kelp species are seriously degraded and the yield is reduced. Therefore, people attach great importance to the collection and preservation of kelp germplasm. Because the generation of kelp gametophytes is short, generally no more than 14 days in the natural state, safe and fast separation of kelp germplasm is a key link in the collection of germplasm. The traditional method of kelp germplasm separation is to select mature and good kelp to grow vegetables, rinse with sterilized seawater, wipe the su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
Inventor 王翔宇刘玮詹冬梅
Owner MARICULTURE INST OF SHANDONG PROVINCE
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