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Solid lipid nanoparticles including elastin-like polypeptides and use thereof

A solid lipid nano and lipid technology, which is applied in the direction of drug delivery, preparations for in vivo tests, non-active ingredients of polymer compounds, etc., can solve the problem of long-term drug consumption

Inactive Publication Date: 2013-08-14
SAMSUNG ELECTRONICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, since SLNs are overstable, drug release can take a long time

Method used

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  • Solid lipid nanoparticles including elastin-like polypeptides and use thereof
  • Solid lipid nanoparticles including elastin-like polypeptides and use thereof
  • Solid lipid nanoparticles including elastin-like polypeptides and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1: Preparation of SLN comprising slightly water-soluble methyl red

[0083] SLNs were prepared using lipids. At room temperature and atmospheric pressure, egg yolk lecithin (PC) as a phospholipid, tricaprin and trilaurin as a triglyceride (the molar ratio of tricaprate:trilaurin is 6 :4) mixture, and cholesteryl oleate as stabilizer are dissolved in the mixed solvent of chloroform and ethanol (volume ratio 2:1) (egg yolk PC: triglyceride: the mol ratio of cholesteryl oleate=5 :2:1). 1-2 ml of the resulting solution was added to a container containing 1 ml of PBS and dispersed by vortexing and sonication, and the organic solvent was evaporated by using a rotary evaporator, and thus, SLNs were formed in 1 ml of PBS (hereinafter , referred to as "control group SLN") because the organic solvent was evaporated. Also, methyl red corresponding to 10% by weight of egg yolk PC was treated in the same manner as above, except that methyl red was mixed with egg yolk PC, ...

Embodiment 2

[0086] Example 2: Preparation of SLN including surfactant

[0087] In the current embodiment, egg yolk PC as a phospholipid, cholesteryl oleate as a stabilizer, and tricaprin and trilaurin as triglycerides (molar ratio 6: The mixture of 4) was dissolved in a mixed solvent of chloroform and ethanol (volume ratio 2:1) at a molar ratio of egg yolk PC:triglyceride:cholesteryl oleate of 5:1:2. 1-2 ml of the resulting solution was added to containers each comprising 1 ml of PBS containing different proportions of the surfactant Tween 20 (0, 2.5, 5 or 10 volume % based on the volume of PBS) and dispersed by vortexing and sonication, and The organic solvent was evaporated by using a rotary evaporator, and thus, SLNs were formed in 1 ml of PBS as the organic solvent was evaporated.

[0088] The average diameter of the resulting SLNs was measured by using a Zetasizer Nano ZS (Malvern instrument, UK), which is a dynamic light scattering (DLS) device. Specifically, 10 μL SLN (1 mg egg y...

Embodiment 3

[0090] Example 3: Preparation of SLNs comprising sparingly water-soluble substances and confirmation using transmission electron microscopy

[0091] In the current embodiment, egg yolk PC as a phospholipid, a mixture of tricaprin and trilaurin (molar ratio 6:4) as a triglyceride, and cholesterol oleate were mixed at room temperature and atmospheric pressure. Dissolve in a mixed solvent of chloroform and ethanol (volume ratio 2:1) with coumarin-6 (10% by weight of egg yolk PC) (which is a slightly water-soluble substance) at a molar ratio of 5:2:1 . 1-2 ml of the resulting solution was added to a container comprising 1 ml of PBS (containing 2% by volume of Tween20, based on the volume of PBS) and dispersed by vortexing and sonication, and the organic solvent was evaporated by using a rotary evaporator, and thus, SLNs were formed in 1 ml PBS as the organic solvent was evaporated.

[0092] The average diameter of the resulting SLNs was measured by using a Zetasizer Nano ZS (Mal...

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Abstract

Solid lipid nanoparticles (SLNs) including elastin-like polypeptides and a use thereof.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of Korean Patent Application No. 10-2012-010505 filed on February 1, 2012 and Korean Patent Application No. 10-2013-0001792 filed on January 7, 2013 at the Korean Intellectual Property Office, The disclosure of which is hereby incorporated by reference in its entirety. technical field [0003] The present disclosure relates to solid lipid nanoparticles (SLN) comprising elastin-like peptide (ELP) polypeptides and uses thereof. Background technique [0004] Solid lipid nanoparticles (SLNs) were developed as an alternative carrier system to emulsions, liposomes, and polymer nanoparticles. SLNs may provide advantages including stabilization of the introduced compound, controlled release, occlusivity, and film formation on the skin (including in vivo effects on the skin). Moreover, when using biological lipids, SLNs provide excellent biocompatibility and biodegradability as well as high...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/42A61K49/00A61K49/18A61K49/14
CPCA61M5/00A61M5/007A61K47/42A61N2/002A61K41/0028A61K9/5123A61K9/5169A61M5/44
Inventor 朴宣玟金泫伶朴在钻蔡洙荣
Owner SAMSUNG ELECTRONICS CO LTD
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