Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Medicago sativaL drought resistance and salt tolerance gene

A drought-resistant and salt-tolerant gene, alfalfa technology, applied in the field of genetic engineering, can solve problems such as the difficulty of realizing stress-resistant varieties and the lack of genetic resources for alfalfa's resistance to stress

Active Publication Date: 2013-07-24
杨凌金栗农业科技有限公司
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, alfalfa lacks stress-resistant genetic resources, and it is not easy to select stress-resistant varieties through conventional cross-breeding

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Medicago sativaL drought resistance and salt tolerance gene
  • Medicago sativaL drought resistance and salt tolerance gene
  • Medicago sativaL drought resistance and salt tolerance gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] The method of the present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0018] The combination of these two methods is expected to obtain a full-length cDNA gene.

[0019] The outline of the 5′RACE steps is as follows: figure 1 Shown: first-strand primer GSP1 annealed to mRNA; mRNA was transcribed into cDNA using SuperScriptIII (invitrogen); RNA was degraded using RNaseH (MBI); cDNA was purified using glass milk; purified cDNA was tailed using dCTP and TdT; dC-tailed cDNA was amplified by PCR with combined anchor primers and nested GSP2; primary PCR products were reamplified using AUAP, UAP, and nested GSP.

[0020] The outline of the 3′RACE steps is as follows: figure 2 Shown: 3′RACE utilizes the naturally occurring Poly(A) tail at the 3′-terminus of mRNA as a universal priming site for PCR amplification, and the mRNAs are first reverse-transcribed with reverse transcriptase and Oligo-dT...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a medicago sativaL drought resistance and salt tolerance gene, which has an amino acid nucleotide sequence represented by SEQ ID NO.1 in the sequence table. After the gene is transformed into medicago sativaL, drought resistance and salt tolerance of the medicago sativaL can be increased, and the strong stress resistance medicago sativaL variety can be rapidly bred with a genetic engineering manner. The strong stress resistance medicago sativaL variety can be obtained by adopting the following process: 1, linking the gene and a plant expression vector pCAMBIA1301 by using T4 ligase to construct a plant expression vector; 2, transforming vector expression plasmid into Agrobacterium tumefaciens GV3101, adopting a leaf disc method to infect tobacco leaves, and adopting hygromycin to screen resistant plants; and 3, transplanting the obtained plants into soil, and observing growth states and phenotypes of the plants.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering and relates to a drought-resistant and salt-tolerant gene of alfalfa. Background technique [0002] Alfalfa (Medicago sativa L.) is the most widely distributed perennial leguminous forage in the world, and the planting area in the world is more than 30 million ha 2 , my country is one of the countries that cultivate and utilize more alfalfa in the world. Because of its rich nutrition, strong palatability, wide cultivation area, and high protein content, it has the reputation of "the king of forage grass" and plays an important role in animal husbandry production. In recent years, due to the increasingly severe climate drought in our country, over-exploitation and extensive management of pastures have greatly restricted the production of alfalfa. Therefore, it is of great practical significance to carry out technical research on improving alfalfa's stress resistance at this stage. How...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/29C12N15/82A01H5/00
Inventor 韩博杨培志呼天明余成群张攀王卫栋张志强曹玉曼杨唯梓
Owner 杨凌金栗农业科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com